ArticleApplied and environmental microbiology2009
Requirement of polyphosphate by Pseudomonas fluorescens Pf0-1 for competitive fitness and heat tolerance in laboratory media and sterile soil.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- Ppx1 putative exopolyphosphatase is essential for polyphosphate accumulation inApplied and environmental microbiology · 2024Article
- The microwave bacteriome: biodiversity of domestic and laboratory microwave ovens.Frontiers in microbiology · 2024Article
- Genetic Basis and Expression Pattern Indicate the Biocontrol Potential and Soil Adaption ofMicroorganisms · 2023Article
- LeveragingFrontiers in microbiology · 2021Review
- The potential for polyphosphate metabolism in Archaea and anaerobic polyphosphate formation in Methanosarcina mazei.Scientific reports · 2019Article
- Alginate genes are required for optimal soil colonization and persistence byAccess microbiology · 2019Article
- [Expression of Proteus mirabilis polyphosphate kinase and preparation of its polyclonal antibodies].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2017Article
- Differential regulation of polyphosphate genes in Pseudomonas aeruginosa.Molecular genetics and genomics : MGG · 2017Article
- Involvement of polyphosphate kinase in virulence and stress tolerance of uropathogenic Proteus mirabilis.Medical microbiology and immunology · 2016Article
- phoU inactivation in Pseudomonas aeruginosa enhances accumulation of ppGpp and polyphosphate.Applied and environmental microbiology · 2015Article
- Importance of positioning for microbial evolution.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Accumulation of inorganic polyphosphate enables stress endurance and catalytic vigour in Pseudomonas putida KT2440.Microbial cell factories · 2013Article
- Colonization strategies of Pseudomonas fluorescens Pf0-1: activation of soil-specific genes important for diverse and specific environments.BMC microbiology · 2013Article
- Fluorometric quantification of polyphosphate in environmental plankton samples: extraction protocols, matrix effects, and nucleic acid interference.Applied and environmental microbiology · 2013Article
- Novel genes involved in Pseudomonas fluorescens Pf0-1 motility and biofilm formation.Applied and environmental microbiology · 2012Article
- Regulation of polyphosphate kinase production by antisense RNA in Pseudomonas fluorescens Pf0-1.Applied and environmental microbiology · 2012Article
- Contribution of TAT system translocated PhoX to Campylobacter jejuni phosphate metabolism and resilience to environmental stresses.PloS one · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Knowledge of the genetic basis for bacterial survival and persistence in soil is a critical component in the development of successful biological control strategies and for understanding the ecological success of bacteria. We found a locus specifying polyphosphate kinase (ppk) and a nonpredicted antisense RNA (iiv8) in Pseudomonas fluorescens Pf0-1 to be necessary for optimal competitive fitness in LB broth culture and sterile loam soil. Pf0-1 lacking ppk and iiv8 was more than 10-fold less competitive against wild-type Pf0-1 in sterile loam soil low in inorganic phosphate. Studies indicated that ppk, and not iiv8, was required for competitive fitness. No role for iiv8 was identified. While a ppk and iiv8 mutant of Pf0-1 did not have increased sensitivity to osmotic, oxidative, and acid stress, it was more sensitive to elevated temperatures in laboratory medium and during growth in sterile soil. ppk was shown to be part of the Pho regulon in P. fluorescens, being upregulated in response to a low external P(i) concentration. Of importance, overproduction of polyphosphate in the soil environment appears to be more deleterious than production of none at all. Our findings reveal a new role for polyphosphate (and the need for proper regulation of its production) in competitive fitness of P. fluorescens in laboratory and soil environments.
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