ArticleApplied and environmental microbiology2009
Species-specific cell wall binding affinity of the S-layer proteins of mosquitocidal bacterium Bacillus sphaericus C3-41.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 12 citations in OpenAlex.
- Adaptation of the binding domain ofFrontiers in microbiology · 2023Article
- Structural basis of cell wall anchoring by SLH domains in Paenibacillus alvei.Nature communications · 2018Article
- Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification.International journal of molecular medicine · 2012Article
- Production, secretion, and cell surface display of recombinant Sporosarcina ureae S-layer fusion proteins in Bacillus megaterium.Applied and environmental microbiology · 2012Article
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4 authors at 1 institution in 1 country.
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Abstract
The binding affinities and specificities of six truncated S-layer homology domain (SLH) polypeptides of mosquitocidal Bacillus sphaericus strain C3-41 with the purified cell wall sacculi have been assayed. The results indicated that the SLH polypeptide comprised of amino acids 31 to 210 was responsible for anchoring the S-layer subunits to the rigid cell wall layer via a distinct type of secondary cell wall polymer and that a motif of the recombinant SLH polypeptide comprising amino acids 152 to 210 (rSLH(152-210)) was essential for the stable binding of the S-layer with the bacterial cell walls. The quantitative assays revealed that the K(D) (equilibrium dissociation constant) values of rSLH(152-210) and rSLH(31-210) with purified cell wall sacculi were 1.11 x 10(-6) M and 1.40 x 10(-6) M, respectively. The qualitative assays demonstrated that the SLH domain of strain C3-41 could bind only to the cell walls or the cells treated with 5 M guanidinium hydrochloride of both toxic and nontoxic B. sphaericus strains but not to those from other bacteria, indicating the species-specific binding of the SLH polypeptide of B. sphaericus with bacterial cell walls.
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