ArticleJournal of bacteriology2009
ResDE-dependent regulation of enterotoxin gene expression in Bacillus cereus: evidence for multiple modes of binding for ResD and interaction with Fnr.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- The major toxins of Bacillus cereus - Current knowledge on distribution, regulation and mechanism of action.EXCLI journal · 2026Review
- Virulence-related genotypic differences amongFrontiers in cellular and infection microbiology · 2023Article
- The Food Poisoning Toxins ofToxins · 2021Review
- Pore-Forming Toxins During Bacterial Infection: Molecular Mechanisms and Potential Therapeutic Targets.Drug design, development and therapy · 2021Review
- TheToxins · 2020Review
- Binding to The Target Cell Surface Is The Crucial Step in Pore Formation of Hemolysin BL fromToxins · 2019Article
- Simulating Intestinal Growth Conditions Enhances Toxin Production of EnteropathogenicFrontiers in microbiology · 2017Article
- Comparative Bioinformatics and Experimental Analysis of the Intergenic Regulatory Regions of Bacillus cereus hbl and nhe Enterotoxin Operons and the Impact of CodY on Virulence Heterogeneity.Frontiers in microbiology · 2016Article
- Adaptation inFrontiers in microbiology · 2016Review
- Cytochrome c551 and the cytochrome c maturation pathway affect virulence gene expression in Bacillus cereus ATCC 14579.Journal of bacteriology · 2015Article
- Nitric oxide as a regulator of B. anthracis pathogenicity.Frontiers in microbiology · 2015Article
- From genome to toxicity: a combinatory approach highlights the complexity of enterotoxin production in Bacillus cereus.Frontiers in microbiology · 2015Article
- Proteomics identifies Bacillus cereus EntD as a pivotal protein for the production of numerous virulence factors.Frontiers in microbiology · 2015Article
- Transcriptional regulation of bacterial virulence gene expression by molecular oxygen and nitric oxide.Virulence · 2014Review
- Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579.PloS one · 2014Article
- Bacillus cereus Fnr binds a [4Fe-4S] cluster and forms a ternary complex with ResD and PlcR.BMC microbiology · 2012Article
- Restricting fermentative potential by proteome remodeling: an adaptive strategy evidenced in Bacillus cereus.Molecular & cellular proteomics : MCP · 2012Article
- From transcriptional landscapes to the identification of biomarkers for robustness.Microbial cell factories · 2011Review
- Lactate dehydrogenase A promotes communication between carbohydrate catabolism and virulence in Bacillus cereus.Journal of bacteriology · 2011Article
- Bacillus cereus cytotoxins Hbl, Nhe and CytK are secreted via the Sec translocation pathway.BMC microbiology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the food-borne pathogen Bacillus cereus F4430/73, the production of major virulence factors hemolysin BL (Hbl) and nonhemolytic enterotoxin (Nhe) is regulated through complex mechanisms. The two-component regulatory system ResDE is involved in the activation of hbl and nhe transcription. Here, the response regulator ResD and the sensor kinase ResE were overexpressed and purified, and autophosphorylation of ResE and transphosphorylation of ResD by ResE were demonstrated in vitro. ResD is mainly monomeric in solution, regardless of its phosphorylation state. ResD was shown to interact directly with promoter regions (p) of the enterotoxin regulator genes resDE, fnr, and plcR and the enterotoxin structural genes nhe and hbl, but with different affinities. Binding of ResD to pplcR, pnhe, and phbl was not dependent on the ResD phosphorylation status. In contrast, ResD phosphorylation significantly increased interactions between ResD and presDE and pfnr. Taken together, these results showed that phosphorylation of ResD results in a different target expression pattern. Furthermore, ResD and the redox activator Fnr were found to physically interact and simultaneously bind their target DNAs. We propose that unphosphorylated ResD acts as an antiactivator of Fnr, while phosphorylated ResD acts as a coactivator of Fnr. Finally, our findings represent the first molecular evidence of the role of ResDE as a sentinel system capable of sensing redox changes and coordinating a response that modulates B. cereus virulence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.