ArticlePlant physiology2009
Antisense down-regulation of the FaPG1 gene reveals an unexpected central role for polygalacturonase in strawberry fruit softening.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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82 citing papers in PubMed, 235 citations in OpenAlex.
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- De Novo Chromosome-Level Genome Assembly of 'Qing Zhou Mi' Landrace Peach and Analysis of Late Maturity and Fruit Weight Traits in Peach.Plants (Basel, Switzerland) · 2026Article
- Advances in Cell Wall Dynamics and Gene Expression in Postharvest Fruit Softening.Plants (Basel, Switzerland) · 2025Review
- Mutation of the Polygalacturonase GeneBiology · 2025Article
- Loss-of-function mutations in the fruit softening geneHorticulture research · 2025Article
- Genome-wide association studies in a diverse strawberry collection unveil loci controlling agronomic and fruit quality traits.The plant genome · 2024Article
- Transcriptome analysis reveals regulatory mechanism of postharvest softening in kiwiberry.BMC plant biology · 2024Article
- Genome-Wide Analysis of Polygalacturonase Gene Family Reveals Its Role in Strawberry Softening.Plants (Basel, Switzerland) · 2024Article
- Fruit softening: evidence for rhamnogalacturonan lyase action in vivo in ripe fruit cell walls.Annals of botany · 2024Article
- Mechanism of abscisic acid in promoting softening of postharvest 'Docteur Jules Guyot' pear (Frontiers in plant science · 2024Article
- Advances in genomics and genome editing for improving strawberry (Frontiers in genetics · 2024Review
- Unraveling cell wall polysaccharides during blueberry ripening: insights into the roles of rhamnogalacturonan-I and arabinogalactan proteins in fruit firmness.Frontiers in plant science · 2024Article
- Foliar spraying of boron prolongs preservation period of strawberry fruits by altering boron form and boron distribution in cell.Frontiers in plant science · 2024Article
- Deciphering the regulatory network of the NAC transcription factor FvRIF, a key regulator of strawberry (Fragaria vesca) fruit ripening.The Plant cell · 2023Article
- Comprehensive Analysis of the Pectate Lyase Gene Family and the Role ofInternational journal of molecular sciences · 2023Article
- CRISPR/Cas9 gene editing technology: a precise and efficient tool for crop quality improvement.Planta · 2023Review
- CRISPR/Cas9 editing of the polygalacturonaseHorticulture research · 2023Article
- Comparative physiological and transcriptomic analyses provide insights into fruit softening in Chinese cherry [Frontiers in plant science · 2023Article
- Abscisic acid-responsive transcription factors PavDof2/6/15 mediate fruit softening in sweet cherry.Plant physiology · 2022Article
- Azacytidine arrests ripening in cultivated strawberry (Fragaria × ananassa) by repressing key genes and altering hormone contents.BMC plant biology · 2022Article
22 more citing papers are in PubMed but not listed here.
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10 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The strawberry (Fragaria x ananassa 'Chandler') fruit undergoes a fast softening during ripening. Polygalacturonase (PG) activity is low during this process, but two ripening-related PG genes, FaPG1 and FaPG2, have been cloned. Both genes were up-regulated during fruit ripening and were also negatively regulated by auxin. To further assess the role of FaPG1 on strawberry softening, transgenic plants containing an antisense sequence of this gene under the control of the 35S promoter (APG lines) were obtained. Sixteen out of 30 independent transgenic lines showed fruit yields similar to those of the control. Several quality parameters were measured in ripe fruits from these 16 lines. Fruit weight was slightly reduced in four lines, and most of them showed an increase in soluble solid content. Half of these lines yielded fruits significantly firmer than did the control. Four APG lines were selected, their ripened fruits being on average 163% firmer than the control. The postharvest softening of APG fruits was also diminished. Ripened fruits from the four selected lines showed a 90% to 95% decrease in FaPG1 transcript abundance, whereas the level of FaPG2 was not significantly altered. Total PG activity was reduced in three of these lines when compared with control fruits. Cell wall extracts from APG fruits showed a reduction in pectin solubilization and an increase in pectins covalently bound to the cell wall. A comparative transcriptomic analysis of gene expression between the ripened receptacle of the control and those of the APG fruits (comprising 1,250 receptacle expressed sequence tags) did not show any statistically significant change. These results indicate that FaPG1 plays a central role in strawberry softening.
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