ArticleThe Journal of biological chemistry2009
Novel interactions of glycosaminoglycans and bacterial glycolipids mediate binding of enterococci to human cells.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 52 citations in OpenAlex.
- Commensal bacterial glycosylation at the interface of host-bacteria interactions.Gut microbes · 2025Review
- The NagY antiterminator inMicrobiology spectrum · 2025Article
- Review
- Enterococcal-host interactions in the gastrointestinal tract and beyond.FEMS microbes · 2024Review
- In Vivo Role of Two-Component Regulatory Systems in Models of Urinary Tract Infections.Pathogens (Basel, Switzerland) · 2023Review
- Ex-Vivo Adhesion ofAnimals : an open access journal from MDPI · 2021Article
- The Role of Glycosaminoglycans in Protection from Neonatal Necrotizing Enterocolitis: A Narrative Review.Nutrients · 2020Review
- The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation.Journal of human lactation : official journal of International Lactation Consultant Association · 2019Article
- The Influence of Heat-KilledFrontiers in microbiology · 2019Article
- Probiotics in human gut microbiota can degrade host glycosaminoglycans.Scientific reports · 2018Article
- Glycointeractions in bacterial pathogenesis.Nature reviews. Microbiology · 2018Review
- Human milk glycosaminoglycans inhibit in vitro the adhesion of Escherichia coli and Salmonella fyris to human intestinal cells.Pediatric research · 2016Article
- Surface Proteoglycans as Mediators in Bacterial Pathogens Infections.Frontiers in microbiology · 2016Review
- Marine Non-Glycosaminoglycan Sulfated Glycans as Potential Pharmaceuticals.Pharmaceuticals (Basel, Switzerland) · 2015Review
- In vitro and in vivo activity of hyperimmune globulin preparations against multiresistant nosocomial pathogens.Infection · 2015Article
- Comprehensive molecular, genomic and phenotypic analysis of a major clone of Enterococcus faecalis MLST ST40.BMC genomics · 2015Article
- The sweet tooth of bacteria: common themes in bacterial glycoconjugates.Microbiology and molecular biology reviews : MMBR · 2014Review
- Crystal structure of a bacterial unsaturated glucuronyl hydrolase with specificity for heparin.The Journal of biological chemistry · 2014Article
- Role of glycolipids in the pathogenesis of Enterococcus faecalis urinary tract infection.PloS one · 2014Article
- Cell-wall glycolipid mutations and their effects on virulence of E. faecalis in a rat model of infective endocarditis.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Enterococcus faecalis is among the most important nosocomial pathogens. The intestinal mucosa is considered to be the main site used by these bacteria for entrance and dissemination. A better understanding of the mechanisms involved in colonization and invasion of enterococci may help to devise methods to prevent infections in hospitalized patients. Glycosaminoglycans, which are present on the surface of all eukaryotic cells, were investigated with regard to their role as host receptors for adhesion of E. faecalis. Competitive binding assays, enzymatic digestion, and reduction of the sulfation of the glycosaminoglycan chains indicated that heparin and heparan sulfate, but not chondroitin sulfate B, played important roles in adhesion of E. faecalis 12030 to Caco2 cells. By using proteinases and carbohydrate oxidation by sodium meta-periodate to modify the bacterial surface, it could be demonstrated that a sugar-containing molecule rather than a protein is the bacterial ligand mediating adhesion to eukaryotic cells. Preincubation of Caco2 cells with the enterococcal glycolipid diglucosyldiacylglycerol but not other carbohydrate cell wall components inhibited bacterial binding. These results may indicate that heparin and/or heparan sulfate on host epithelial cells and diglucosyldiacylglycerol, either itself or as a partial structure of lipoteichoic acid, are involved in enterococcal adhesion to colonic epithelia, the first step in translocation from the intestinal tract.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.