ArticleMolecular cell2009
An alpha motif at Tas3 C terminus mediates RITS cis spreading and promotes heterochromatic gene silencing.
Article in Molecular cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
- Stress-sensitive dynamics of miRNAs and Elba1 in Drosophila embryogenesis.Molecular systems biology · 2023Article
- Challenging dogmas: How transgenerational epigenetics reshapes our views on life.Journal of experimental zoology. Part A, Ecological and integrative physiology · 2022Article
- piRNA- and siRNA-mediated transcriptional repression in Drosophila, mice, and yeast: new insights and biodiversity.EMBO reports · 2021Review
- Molecular principles of Piwi-mediated cotranscriptional silencing through the dimeric SFiNX complex.Genes & development · 2021Article
- TASOR is a pseudo-PARP that directs HUSH complex assembly and epigenetic transposon control.Nature communications · 2020Article
- Set1/COMPASS repels heterochromatin invasion at euchromatic sites by disrupting Suv39/Clr4 activity and nucleosome stability.Genes & development · 2020Article
- RNA-induced initiation of transcriptional silencing (RITS) complex structure and function.RNA biology · 2019Review
- Article
- Nucleation and spreading of a heterochromatic domain in fission yeast.Nature communications · 2016Article
- A Novel Epigenetic Silencing Pathway Involving the Highly Conserved 5'-3' Exoribonuclease Dhp1/Rat1/Xrn2 in Schizosaccharomyces pombe.PLoS genetics · 2016Article
- H3K9 methylation extends across natural boundaries of heterochromatin in the absence of an HP1 protein.The EMBO journal · 2015Article
- Regulation of histone methylation by noncoding RNAs.Biochimica et biophysica acta · 2014Review
- DNA replication components as regulators of epigenetic inheritance--lesson from fission yeast centromere.Protein & cell · 2014Review
- Mediator directs co-transcriptional heterochromatin assembly by RNA interference-dependent and -independent pathways.PLoS genetics · 2013Article
- The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS.Nature structural & molecular biology · 2011Article
- Phylogenetic comparison of small RNA-triggered transcriptional gene silencing.The Journal of biological chemistry · 2011Review
- S. pombe replication protein Cdc18 (Cdc6) interacts with Swi6 (HP1) heterochromatin protein: region specific effects and replication timing in the centromere.Cell cycle (Georgetown, Tex.) · 2011Article
- The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation.Molecular cell · 2010Article
- Chromatin higher-order structure and dynamics.Cold Spring Harbor perspectives in biology · 2010Review
- UTX mediates demethylation of H3K27me3 at muscle-specific genes during myogenesis.The EMBO journal · 2010Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
RNA interference (RNAi) plays a pivotal role in the formation of heterochromatin at the fission yeast centromeres. The RNA-induced transcriptional silencing (RITS) complex, composed of heterochromatic small interfering RNAs (siRNAs), the siRNA-binding protein Ago1, the chromodomain protein Chp1, and the Ago1/Chp1-interacting protein Tas3, provides a physical tether between the RNAi and heterochromatin assembly pathways. Here, we report the structural and functional characterization of a C-terminal Tas3 alpha-helical motif (TAM), which self-associates into a helical polymer and is required for cis spreading of RITS in centromeric DNA regions. Site-directed mutations of key residues within the hydrophobic monomer-monomer interface disrupt Tas3-TAM polymeric self-association in vitro and result in loss of gene silencing, spreading of RITS, and a dramatic reduction in centromeric siRNAs in vivo. These results demonstrate that, in addition to the chromodomain of Chp1 and siRNA-loaded Ago1, Tas3 self-association is required for RITS spreading and efficient heterochromatic gene silencing at centromeric repeat regions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.