ArticleJournal of neuroscience methods2009
An efficient and reproducible method for quantifying macrophages in different experimental models of central nervous system pathology.
Article in Journal of neuroscience methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
80 citing papers in PubMed, 1 synthesis or guideline pooled it, 127 citations in OpenAlex.
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- Repeated mild closed head injury in neonatal rats results in sustained cognitive deficits associated with chronic microglial activation and neurodegeneration.Journal of neuropathology and experimental neurology · 2023Article
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- A method for the selective depletion of microglia in the dorsal hippocampus in the juvenile rat brain.Journal of neuroscience methods · 2022Article
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- Minocycline attenuation of rat corpus callosum abnormality mediated by low-dose lipopolysaccharide-induced microglia activation.Journal of neuroinflammation · 2021Article
- Role of age and neuroinflammation in the mechanism of cognitive deficits in sickle cell disease.Experimental biology and medicine (Maywood, N.J.) · 2021Article
- Brain region-dependent alterations in polysialic acid immunoreactivity across the estrous cycle in mice.Hormones and behavior · 2020Article
- One proline deletion in the fusion peptide of neurotropic mouse hepatitis virus (MHV) restricts retrograde axonal transport and neurodegeneration.The Journal of biological chemistry · 2020Article
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- Docosahexaenoic acid decreased neuroinflammation in rat pups after controlled cortical impact.Experimental neurology · 2019Article
- Tracking the evolution of CNS remyelinating lesion in mice with neutral red dye.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Dim light at night impairs recovery from global cerebral ischemia.Experimental neurology · 2019Article
- A proline insertion-deletion in the spike glycoprotein fusion peptide of mouse hepatitis virus strongly alters neuropathology.The Journal of biological chemistry · 2019Article
- Repeated social defeat in female mice induces anxiety-like behavior associated with enhanced myelopoiesis and increased monocyte accumulation in the brain.Brain, behavior, and immunity · 2019Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Historically, microglia/macrophages are quantified in the pathological central nervous system (CNS) by counting cell profiles then expressing the data as cells/mm(2). However, because it is difficult to visualize individual cells in dense clusters and in most cases it is unimportant to know the absolute number of macrophages within lesioned tissue, alternative methods may be more efficient for quantifying the magnitude of the macrophage response in the context of different experimental variables (e.g., therapeutic intervention or time post-injury/infection). The present study provides the first in-depth comparison of different techniques commonly used to quantify microglial/macrophage reactions in the pathological spinal cord. Individuals from the same and different laboratories applied techniques of digital image analysis (DIA), standard cell profile counting and a computer-assisted cell counting method with unbiased sampling to quantify macrophages in focal inflammatory lesions, disseminated lesions caused by autoimmune inflammation or at sites of spinal trauma. Our goal was to find a simple, rapid and sensitive method with minimal variability between trials and users. DIA was consistently the least variable and most time-efficient method for assessing the magnitude of macrophage responses across lesions and between users. When used to evaluate the efficacy of an anti-inflammatory treatment, DIA was 5-35 x faster than cell counting and was sensitive enough to detect group differences while eliminating inter-user variability. Since lesions are clearly defined and single profiles of microglia/macrophages are difficult to discern in most pathological specimens of brain or spinal cord, DIA offers significant advantages over other techniques for quantifying activated macrophages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.