ArticlePlant methods2009
Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues.
Article in Plant methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 76 citations in OpenAlex.
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- Exploring the Significance, Extraction, and Characterization of Plant-Derived Secondary Metabolites in Complex Mixtures.Metabolites · 2024Article
- Proteomic and Low-Polar Metabolite Profiling Reveal Unique Dynamics in Fatty Acid Metabolism during Flower and Berry Development of Table Grapes.International journal of molecular sciences · 2023Article
- Phytosterols Are Involved in Sclareol-Induced Chlorophyll Reductions inPlants (Basel, Switzerland) · 2023Article
- Free and Esterified Tocopherols, Tocotrienols and Other Extractable and Non-Extractable Tocochromanol-Related Molecules: Compendium of Knowledge, Future Perspectives and Recommendations for Chromatographic Techniques, Tools, and Approaches Used for Tocochromanol Determination.Molecules (Basel, Switzerland) · 2022Review
- Suitability of Solvent-Assisted Extraction for Recovery of Lipophilic Phytochemicals in Sugarcane Straw and Bagasse.Foods (Basel, Switzerland) · 2022Article
- Chemical Profiling of Two ItalianPlants (Basel, Switzerland) · 2022Article
- Species-independent analytical tools for next-generation agriculture.Nature plants · 2020Review
- Antioxidant Activities ofAntioxidants (Basel, Switzerland) · 2018Article
- TheFrontiers in plant science · 2018Article
- A comparison of semi-quantitative methods suitable for establishing volatile profiles.Plant methods · 2018Article
- Metabolite Profiling of Eastern Teaberry (Gaultheria procumbens L.) Lipophilic Leaf Extracts with Hyaluronidase and Lipoxygenase Inhibitory Activity.Molecules (Basel, Switzerland) · 2017Article
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- Tetracycline uptake and metabolism by vetiver grass (Chrysopogon zizanioides L. Nash).Environmental science and pollution research international · 2016Article
- Metabolite profiling of Dioscorea (yam) species reveals underutilised biodiversity and renewable sources for high-value compounds.Scientific reports · 2016Article
- Analysis of phenolic and cyclic compounds in plants using derivatization techniques in combination with GC-MS-based metabolite profiling.Molecules (Basel, Switzerland) · 2015Article
- Strategies for vitamin B6 biofortification of plants: a dual role as a micronutrient and a stress protectant.Frontiers in plant science · 2013Article
- Tomato fruit photosynthesis is seemingly unimportant in primary metabolism and ripening but plays a considerable role in seed development.Plant physiology · 2011Article
- Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe concept of metabolite profiling has been around for decades and technical innovations are now enabling it to be carried out on a large scale with respect to the number of both metabolites measured and experiments carried out. However, studies are generally confined to polar compounds alone. Here we describe a simple method for lipophilic compounds analysis in various plant tissues.
resultsWe choose the same preparative and instrumental platform for lipophilic profiling as that we routinely use for polar metabolites measurements. The method was validated in terms of linearity, carryover, reproducibility and recovery rates, as well as using various plant tissues.As a first case study we present metabolic profiling of Arabidopsis root and shoot tissue of wild type (C24) and mutant (rsr4-1) plants deficient on vitamin B6. We found significant alterations in lipid constituent contents, especially in the roots, which were characterised by dramatic increases in several fatty acids, thus providing further hint for the role of pyridoxine in oxidative stress and lipid peroxidation.The second example is the lipophilic profiling of red and green tomato fruit cuticles of wild type (Alisa Craig) and the DFD (delayed fruit deterioration) mutant, which we compared and contrasted with the more focused wax analysis of these plants reported before.
conclusionWe can rapidly and reliably detect and quantify over 40 lipophilic metabolites including fatty acids, fatty alcohols, alkanes, sterols and tocopherols. The method presented here affords a simple and rapid, yet robust complement to previously validated methods of polar metabolite profiling by gas-chromatography mass-spectrometry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.