ArticleJournal of translational medicine2009
Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels.
Article in Journal of translational medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Specific Circular RNA Signature of Endothelial Cells: Potential Implications in Vascular Pathophysiology.International journal of molecular sciences · 2024Article
- UV-B Filter Octylmethoxycinnamate Alters the Vascular Contractility Patterns in Pregnant Women with Hypothyroidism.Biomedicines · 2021Article
- ITE Suppresses Angiogenic Responses in Human Artery and Vein Endothelial Cells: Differential Roles of AhR.Reproductive toxicology (Elmsford, N.Y.) · 2017Article
- Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells.Journal of visualized experiments : JoVE · 2017Article
- 2,3,7,8-Tetrachlorodibenzo-p-dioxin differentially suppresses angiogenic responses in human placental vein and artery endothelial cells.Toxicology · 2015Article
- Human umbilical endothelial cells (HUVECs) have a sex: characterisation of the phenotype of male and female cells.Biology of sex differences · 2014Article
- Metabolomic profiling in blood from umbilical cords of low birth weight newborns.Journal of translational medicine · 2012Article
- Cryopreservation of human vascular umbilical cord cells under good manufacturing practice conditions for future cell banks.Journal of translational medicine · 2012Article
- Hypoxia stimulates the expression of macrophage migration inhibitory factor in human vascular smooth muscle cells via HIF-1alpha dependent pathway.BMC cell biology · 2010Article
- Angiotensin II upregulates the expression of placental growth factor in human vascular endothelial cells and smooth muscle cells.BMC cell biology · 2010Article
- Early vascular phenotypes in the genesis of hypertension.Pediatric nephrology (Berlin, Germany) · 2010Review
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Authors and funding
6 authors.
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Abstract
backgroundLow birth weight has been related to an increased risk for developing high blood pressure in adult life. The molecular and cellular analysis of umbilical cord artery and vein may provide information about the early vascular characteristics of an individual. We have assessed several phenotype characteristics of the four vascular cell types derived from human umbilical cords of newborns with different birth weight. Further follow-up studies could show the association of those vascular properties with infancy and adulthood blood pressure.
methodsEndothelial and smooth muscle cell cultures were obtained from umbilical cords from two groups of newborns of birth weight less than 2.8 kg or higher than 3.5 kg. The expression of specific endothelial cell markers (von Willebrand factor, CD31, and the binding and internalization of acetylated low-density lipoprotein) and the smooth muscle cell specific alpha-actin have been evaluated. Cell culture viability, proliferation kinetic, growth fraction (expression of Ki67) and percentage of senescent cells (detection of beta-galactosidase activity at pH 6.0) have been determined. Endothelial cell projection area was determined by morphometric analysis of cell cultures after CD31 immunodetection.
resultsThe highest variation was found in cell density at the confluence of endothelial cell cultures derived from umbilical cord arteries (66,789 +/- 5,093 cells/cm(2) vs. 45,630 +/- 11,927 cells/cm(2), p < 0.05). Morphometric analysis indicated that the projection area of the artery endothelial cells (1,161 +/- 198 and 1,544 +/- 472 microm(2), p < 0.05), but not those derived from the vein from individuals with a birth weight lower than 2.8 kg was lower than that of cells from individuals with a birth weight higher than 3.5 kg.
conclusionThe analysis of umbilical cord artery endothelial cells, which demonstrated differences in cell size related to birth weight, can provide hints about the cellular and molecular links between lower birth weight and increased adult high blood pressure risk.
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