Evidence map›Paper›PMID 19392979›Full record

ArticleAmerican journal of reproductive immunology (New York, N.Y. : 1989)2009

Suppression of Mamu-AG by RNA interference.

Jessica G Drenzek, Jolanta Vidiguriene, Geminis Vidiguris, Richard L Grendell, Svetlana V Dambaeva, Maureen Durning, Thaddeus G Golos

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of reproductive immunology (New York, N.Y. : 1989), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Nanoparticles targeting HLA-G for gene therapy in cancer.Medical oncology (Northwood, London, England) · 2012
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jessica G DrenzekDepartment of Obstetrics and Gynecology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53715-1299, USA.
Jolanta Vidiguriene
Geminis Vidiguris
Richard L Grendell
Svetlana V Dambaeva
Maureen Durning
Thaddeus G Golos

Funding

ZOLEDRONATE PREVENTS BONE LOSS IN OVARIECTOMIZED RHESUS MONKEYSP51RR000167 · NCRR · UNIVERSITY OF WISCONSIN-MADISON · PI WATKINS, DAVID I · 1985 to 2011
$106.7M
EXTRAMURAL RES FACIL CONST: REPRODUCTIVE, GROWTH &DVMT, MENOPAUSEC06RR020141 · NCRR · UNIVERSITY OF WISCONSIN MADISON · PI CADWALLADER, MARTIN · 2004 to 2004
$3.6M
Endocrinology-Reproductive Physiology Training GrantT32HD041921 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI BIRD, IAN M. · 2004 to 2022
$3.0M
PRIMATE MODEL OF HUMAN MATERNAL FETAL IMMUNE TOLERANCER01HD034215 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI GOLOS, THADDEUS G · 1995 to 2006
$2.6M
EXTRAMURAL REGIONAL FACILITIES CONSTRUCTIONC06RR015459 · NCRR · UNIVERSITY OF WISCONSIN MADISON · PI CADWALLADER, MARTIN · 2000 to 2000
$1.9M
Primate Endometrial Responses to Placental MHC Class I MoleculesR21AI076734 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI GOLOS, THADDEUS G · 2008 to 2009
$360k
NCRR NIH HHS C06 RR015459NCRR NIH HHS C06 RR020141NCRR NIH HHS P51 RR000167NCRR NIH HHS RR020141-01NCRR NIH HHS RR15459-01NIAID NIH HHS R21 AI076734NICHD NIH HHS R01 HD034215NICHD NIH HHS R01 HD34215NICHD NIH HHS T32 HD041921
6 · The paper itself

Abstract

problemThe role of placental major histocompatibility complex (MHC) class I molecules in pregnancy is not well understood. Mamu-AG, the rhesus monkey homology of human leukocyte antigen (HLA)-G expressed in the human placenta, was targeted for degradation by RNA interference (RNAi), a powerful tool to aid in determining gene function, to determine the effect that this knockdown has on NK cell function. METHOD OF STUDY: A series of potential target short hairpin RNA (shRNA) sequences to suppress Mamu-AG expression was screened, which identified an optimal sequence to use in transfection experiments. Knockdown in two different Mamu-AG-expressing cell lines was measured by flow cytometry. Cytotoxicity assays were performed to correlate Mamu-AG expression with NK cell cytotoxicity.

resultsDecreased expression of Mamu-AG by short interfering RNA (siRNA) (70-80%) in cell types tested was associated with increased lysis of Mamu-AG target cells.

conclusionTarget sequences have been identified that knocked down Mamu-AG expression by RNAi and increased lysis by NK cells. This supports the concept that NK cell receptors recognize this placental non-classical MHC class I molecule.

Indexed as

RNA InterferenceAnimalsCell LineFemaleHistocompatibility Antigens Class IHumansImmunity, CellularKiller Cells, NaturalMacaca mulattaPlacentaPregnancyHistocompatibility Antigens Class I

Identifiers

PMID19392979
PMCPMC2889004

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.