ArticleScandinavian journal of gastroenterology2009
Gene expression in Barrett's esophagus: laser capture versus whole tissue.
Article in Scandinavian journal of gastroenterology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
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- Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue.Journal of visualized experiments : JoVE · 2020Article
- Preventing Metachronous Gastric Cancer after the Endoscopic Resection of Gastric Epithelial Neoplasia: Roles ofGut and liver · 2020Review
- The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow.Clinical proteomics · 2020Article
- DNA methylation silencing of microRNA gene methylator in the precancerous background mucosa with and without gastric cancer: Analysis of the effects of H. pylori eradication and long-term aspirin use.Scientific reports · 2019Article
- Computer-Aided Laser Dissection: A Microdissection Workflow Leveraging Image Analysis Tools.Journal of pathology informatics · 2018Article
- Quantitative proteomics analysis of mitochondrial proteins in lung adenocarcinomas and normal lung tissue using iTRAQ and tandem mass spectrometry.American journal of translational research · 2017Article
- Site-specific gene expression profiling as a novel strategy for unravelling keloid disease pathobiology.PloS one · 2017Article
- Localization of specialized intestinal metaplasia and the molecular alterations in Barrett esophagus in a Japanese population: an analysis of biopsy samples based on the "Seattle" biopsy protocol.Human pathology · 2016Article
- Impact of upfront cellular enrichment by laser capture microdissection on protein and phosphoprotein drug target signaling activation measurements in human lung cancer: Implications for personalized medicine.Proteomics. Clinical applications · 2015Article
- Viral expression and molecular profiling in liver tissue versus microdissected hepatocytes in hepatitis B virus-associated hepatocellular carcinoma.Journal of translational medicine · 2014Article
- SIVQ-LCM protocol for the ArcturusXT instrument.Journal of visualized experiments : JoVE · 2014Article
- A mill based instrument and software system for dissecting slide-mounted tissue that provides digital guidance and documentation.BMC clinical pathology · 2013Article
- Article
- EGFR and KRAS mutation analysis in cytologic samples of lung adenocarcinoma enabled by laser capture microdissection.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2012Article
- MS4A1 dysregulation in asbestos-related lung squamous cell carcinoma is due to CD20 stromal lymphocyte expression.PloS one · 2012Article
- Genetic dissection of eosinophilic esophagitis provides insight into disease pathogenesis and treatment strategies.The Journal of allergy and clinical immunology · 2011Review
- Why is it crucial to reintegrate pathology into cancer research?BioEssays : news and reviews in molecular, cellular and developmental biology · 2011Article
- SIVQ-aided laser capture microdissection: A tool for high-throughput expression profiling.Journal of pathology informatics · 2011Article
- MicroRNA analysis of microdissected normal squamous esophageal epithelium and tumor cells.American journal of cancer research · 2011Article
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Authors and funding
8 authors.
Funding
Abstract
objectiveDiagnosis of Barrett's esophagus (BE) is typically done through morphologic analysis of esophageal tissue biopsy. Such samples contain several cell types. Laser capture microdissection (LCM) allows the isolation of specific cells from heterogeneous cell populations. The purpose of this study was to determine the degree of overlap of the two sample types and to define a set of genes that might serve as biochemical markers for BE. MATERIAL AND
methodsBiopsies were obtained from regions of the glandular tissue of BE and normal esophagus from 9 subjects with BE. Samples from 5 subjects were examined as whole tissue (BE [whole]; E [whole]), and in 4 subjects the glandular epithelium of BE was isolated using LCM (BE [LCM]) and compared with the averaged values (E [LCM]) for both basal cell (B [LCM]) and squamous cell (S [LCM]) epithelium.
resultsGene expression revealed 1797 probe sets between BE [whole] and E [whole] (fold change > 2.0; p<0.001). Most of these genes (74%) were also differentially expressed between BE [LCM] and E [LCM], showing that there was high concordance between the two sampling methods. LCM provided a great deal of additional information (2113 genes) about the alterations in gene expression that may represent the BE phenotype.
conclusionsThere are differences in gene expression profiles depending on whether specimens are whole tissue biopsies or LCM dissected. Whole tissue biopsies should prove satisfactory for diagnostic purposes. Because the data from LCM samples delineated many more Barrett's-specific genes, this procedure might provide more information regarding pathogenesis than would whole tissue material.
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