ArticleMolecular vision2009
Release of 11-cis-retinal from cellular retinaldehyde-binding protein by acidic lipids.
Article in Molecular vision, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- Chromophore-loaded CRALBP mutant proteins restore rod function in chromophore-deficient mice.Molecular therapy. Advances · 2026Article
- Mechanisms and Functions of Chromophore Regeneration in the Classical Visual Cycle: Implications for Retinal Disease Pathogenesis and Therapy.Biomolecules · 2025Review
- The molecular mechanisms of visual chromophore release from cellular retinaldehyde-binding protein.Structure (London, England : 1993) · 2025Article
- The Amphipathic Helix in Visual Cycle Proteins: A Review.Advances in experimental medicine and biology · 2023Review
- The Role of Vitamin A in Retinal Diseases.International journal of molecular sciences · 2022Review
- The molecular aspects of absorption and metabolism of carotenoids and retinoids in vertebrates.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2020Review
- Chloroplast Sec14-like 1 (CPSFL1) is essential for normal chloroplast development and affects carotenoid accumulation inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Sec14 domain protein is required for photoautotrophic growth and chloroplast vesicle formation inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Lipid Exchangers: Cellular Functions and Mechanistic Links With Phosphoinositide Metabolism.Frontiers in cell and developmental biology · 2020Review
- Lipid transfer proteins: the lipid commute via shuttles, bridges and tubes.Nature reviews. Molecular cell biology · 2019Review
- Structural biology of 11-The Biochemical journal · 2018Review
- Characterization of Human iPSC-RPE on a Prosthetic Bruch's Membrane Manufactured From Silk Fibroin.Investigative ophthalmology & visual science · 2018Article
- Enhancing Understanding of the Visual Cycle by Applying CRISPR/Cas9 Gene Editing in Zebrafish.Frontiers in cell and developmental biology · 2018Review
- Allosteric modulation of the substrate specificity of acyl-CoA wax alcohol acyltransferase 2.Journal of lipid research · 2017Article
- CRALBP supports the mammalian retinal visual cycle and cone vision.The Journal of clinical investigation · 2015Article
- Mechanisms of recognition and binding of α-TTP to the plasma membrane by multi-scale molecular dynamics simulations.Frontiers in molecular biosciences · 2015Article
- Identification of the 11-cis-specific retinyl-ester synthase in retinal Müller cells as multifunctional O-acyltransferase (MFAT).Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Structural approaches to understanding retinal proteins needed for vision.Current opinion in cell biology · 2014Review
- Chemistry of the retinoid (visual) cycle.Chemical reviews · 2014Review
- Identification of DES1 as a vitamin A isomerase in Müller glial cells of the retina.Nature chemical biology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo determine molecular mechanisms for the release of 11-cis-retinal from the binding pocket of cellular retinaldehyde-binding protein (CRALBP).
methodsBinding of CRALBP to lipid surfaces was assessed with a lipid-immunoblot assay. Lipids were presented to CRALBP as small unilamellar vesicles (SUVs) consisting of phosphatidylcholine (PC) plus other lipids. Release of 9-cis-retinal or 11-cis-retinal from CRALBP was measured with spectral and high performance liquid chromatography (HPLC) assays based on the protection of the protein-bound retinal carbonyl group from reaction with NH(2)OH. The electrostatic surface potential of CRALBP was calculated from a model of its structure using the program CCP4mg.
resultsIncubation of CRALBP.11-cis-retinal with lipids absorbed on nitrocellulose revealed binding to the acidic lipids, phosphatidic acid (PA)>phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P(3)]>phosphatidylserine (PS)> PI(4,5)P(2) and little or no binding to PC, phosphatidylethanolamine (PE), or PI(4)P. 11-cis-retinal was released during incubation of CRALBP with SUVs consisting of PC plus 50 mol% PA but not during incubation with those composed of 100 mol% PC. The efficacy of release of 9-cis-retinal or 11-cis-retinal from CRALBP by phospholipid-containing SUVs generally paralleled that of the binding of CRALBP to the lipids (PA>PS>PI>>PC). Examination of the electrostatic surface potential of the protein structure revealed a basic recess on one face of the protein, which may bind acidic lipids.
conclusionsOur results identify the first physiologic substances that release 11-cis-retinal from CRALBP. PA and PS are relatively minor membrane lipids that can be generated in the cytoplasmic leaflet of the plasma membrane in response to various signal transduction pathways, where they could interact with cytosolic CRALBP. The mechanism for release of retinal from CRALBP by acidic lipids remains to be determined but could involve binding of the acidic lipid in the 11-cis-retinal binding site or to the positive basic recess on the protein surface. These results open a new facet in our understanding of how CRALBP functions in the regeneration of visual pigments.
Indexed as
Identifiers
19390642PMC2672148W1842330439What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.