ArticleJournal of visualized experiments : JoVE2009
High-resolution video tracking of locomotion in adult Drosophila melanogaster.
Article in Journal of visualized experiments : JoVE, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Artificial gravity partially protects space-induced neurological deficits in Drosophila melanogaster.Cell reports · 2022Article
- EasyFlyTracker: A Simple Video Tracking Python Package for Analyzing AdultFrontiers in behavioral neuroscience · 2021Article
- Behavior Individuality: A Focus onFrontiers in physiology · 2021Review
- Small Animal Video Tracking for Activity and Path Analysis Using a Novel Open-Source Multi-Platform Application (AnimApp).Scientific reports · 2019Article
- Quantifying and predicting Drosophila larvae crawling phenotypes.Scientific reports · 2016Article
- Impaired climbing and flight behaviour in Drosophila melanogaster following carbon dioxide anaesthesia.Scientific reports · 2015Article
- Straightforward assay for quantification of social avoidance in Drosophila melanogaster.Journal of visualized experiments : JoVE · 2014Article
- Acquisition of high-quality digital video of Drosophila larval and adult behaviors from a lateral perspective.Journal of visualized experiments : JoVE · 2014Article
- Methods to characterize spontaneous and startle-induced locomotion in a rotenone-induced Parkinson's disease model of Drosophila.Journal of visualized experiments : JoVE · 2014Article
- Determination of the spontaneous locomotor activity in Drosophila melanogaster.Journal of visualized experiments : JoVE · 2014Article
- A low-cost method for analyzing seizure-like activity and movement in Drosophila.Journal of visualized experiments : JoVE · 2014Article
- Regulation of dopamine release by CASK-β modulates locomotor initiation in Drosophila melanogaster.Frontiers in behavioral neuroscience · 2014Article
- Quantification of gait parameters in freely walking wild type and sensory deprived Drosophila melanogaster.eLife · 2013Article
- Methods to assay Drosophila behavior.Journal of visualized experiments : JoVE · 2012Article
- A simple assay to study social behavior in Drosophila: measurement of social space within a group.Genes, brain, and behavior · 2012Article
- Dopamine modulates the rest period length without perturbation of its power law distribution in Drosophila melanogaster.PloS one · 2012Article
- The ALS-associated proteins FUS and TDP-43 function together to affect Drosophila locomotion and life span.The Journal of clinical investigation · 2011Article
- Central regulation of locomotor behavior of Drosophila melanogaster depends on a CASK isoform containing CaMK-like and L27 domains.Genetics · 2011Article
- Alzheimer's disease: insights from Drosophila melanogaster models.Trends in biochemical sciences · 2010Review
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Authors and funding
3 authors.
Funding
Abstract
Flies provide an important model for studying complex behavior due to the plethora of genetic tools available to researchers in this field. Studying locomotor behavior in Drosophila melanogaster relies on the ability to be able to quantify changes in motion during or in response to a given task. For this reason, a high-resolution video tracking system, such as the one we describe in this paper, is a valuable tool for measuring locomotion in real-time. Our protocol involves the use of an initial air pulse to break the flies momentum, followed by a thirty second filming period in a square chamber. A tracking program is then used to calculate the instantaneous speed of each fly within the chamber in 10 msec increments. Analysis software then compiles this data, and outputs a variety of parameters such as average speed, max speed, time spent in motion, acceleration, etc. This protocol will discuss proper feeding and management of flies for behavioral tasks, handling flies without anesthetization or immobilization, setting up a controlled environment, and running the assay from start to finish.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.