ArticleRNA (New York, N.Y.)2009
An extra double-stranded RNA binding domain confers high activity to a squid RNA editing enzyme.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- Structural perspectives on adenosine to inosine RNA editing by ADARs.Molecular therapy. Nucleic acids · 2024Review
- Squid express conserved ADAR orthologs that possess novel features.Frontiers in genome editing · 2023Article
- Genome and transcriptome mechanisms driving cephalopod evolution.Nature communications · 2022Article
- Review
- To protect and modify double-stranded RNA - the critical roles of ADARs in development, immunity and oncogenesis.Critical reviews in biochemistry and molecular biology · 2021Review
- Spatially regulated editing of genetic information within a neuron.Nucleic acids research · 2020Article
- Current strategies for Site-Directed RNA Editing using ADARs.Methods (San Diego, Calif.) · 2019Review
- A-to-I mRNA editing in fungi: occurrence, function, and evolution.Cellular and molecular life sciences : CMLS · 2019Review
- A-to-I RNA editing - immune protector and transcriptome diversifier.Nature reviews. Genetics · 2018Review
- The evolution and adaptation of A-to-I RNA editing.PLoS genetics · 2017Review
- Article
- An efficient system for selectively altering genetic information within mRNAs.Nucleic acids research · 2016Article
- Article
- The emerging role of RNA editing in plasticity.The Journal of experimental biology · 2015Review
- The majority of transcripts in the squid nervous system are extensively recoded by A-to-I RNA editing.eLife · 2015Article
- Regulation of Ion Channel and Transporter Function Through RNA Editing.Current issues in molecular biology · 2015Review
- Correction of mutations within the cystic fibrosis transmembrane conductance regulator by site-directed RNA editing.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The ADAR protein family.Genome biology · 2012Review
- A role for A-to-I RNA editing in temperature adaptation.Physiology (Bethesda, Md.) · 2012Review
- Genes come and go: the evolutionarily plastic path of budding yeast RNase III enzymes.RNA biology · 2012Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
RNA editing by adenosine deamination is particularly prevalent in the squid nervous system. We hypothesized that the squid editing enzyme might contain structural differences that help explain this phenomenon. As a first step, a squid adenosine deaminase that acts on RNA (sqADAR2a) cDNA and the gene that encodes it were cloned from the giant axon system. PCR and RNase protection assays showed that a splice variant of this clone (sqADAR2b) was also expressed in this tissue. Both versions are homologous to the vertebrate ADAR2 family. sqADAR2b encodes a conventional ADAR2 family member with an evolutionarily conserved deaminase domain and two double-stranded RNA binding domains (dsRBD). sqADAR2a differs from sqADAR2b by containing an optional exon that encodes an "extra" dsRBD. Both splice variants are expressed at comparable levels and are extensively edited, each in a unique pattern. Recombinant sqADAR2a and sqADAR2b, produced in Pichia pastoris, are both active on duplex RNA. Using a standard 48-h protein induction, both sqADAR2a and sqADAR2b exhibit promiscuous self-editing; however, this activity is particularly robust for sqADAR2a. By decreasing the induction time to 16 h, self-editing was mostly eliminated. We next tested the ability of sqADAR2a and sqADAR2b to edit two K+ channel mRNAs in vitro. Both substrates are known to be edited in squid. For each mRNA, sqADAR2a edited many more sites than sqADAR2b. These data suggest that the "extra" dsRBD confers high activity on sqADAR2a.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.