ArticleDevelopmental biology2009
Phospholipase D1 is required for angiogenesis of intersegmental blood vessels in zebrafish.
Article in Developmental biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Identification of Growth-Related SNPs and Genes Through Genome-Wide Association Studies in Pseudobagrus ussuriensis.Marine biotechnology (New York, N.Y.) · 2026Article
- NADInternational journal of molecular sciences · 2025Article
- Article
- Mammalian phospholipase D: Function, and therapeutics.Progress in lipid research · 2020Review
- Functional analysis of mammalian phospholipase D enzymes.Bioscience reports · 2018Article
- Rapid and Integrative Discovery of Retina Regulatory Molecules.Cell reports · 2018Article
- Proliferative and metastatic roles for Phospholipase D in mouse models of cancer.Advances in biological regulation · 2018Review
- Systematic Functional Testing of Rare Variants: Contributions of CFI to Age-Related Macular Degeneration.Investigative ophthalmology & visual science · 2017Article
- Loss of phospholipase D2 impairs VEGF-induced angiogenesis.BMB reports · 2016Article
- Angiogenic gene signature in human pancreatic cancer correlates with TGF-beta and inflammatory transcriptomes.Oncotarget · 2016Article
- Phospholipase D signaling pathways and phosphatidic acid as therapeutic targets in cancer.Pharmacological reviews · 2014Review
- Functional regulation of phospholipase D expression in cancer and inflammation.The Journal of biological chemistry · 2014Review
- Cellular and physiological roles for phospholipase D1 in cancer.The Journal of biological chemistry · 2014Review
- Zebrafish yolk lipid processing: a tractable tool for the study of vertebrate lipid transport and metabolism.Disease models & mechanisms · 2014Article
- Zebrafish WNK lysine deficient protein kinase 1 (wnk1) affects angiogenesis associated with VEGF signaling.PloS one · 2014Article
- A functional variant in the CFI gene confers a high risk of age-related macular degeneration.Nature genetics · 2013Article
- Key roles for the lipid signaling enzyme phospholipase d1 in the tumor microenvironment during tumor angiogenesis and metastasis.Science signaling · 2012Article
- Phospholipase signalling networks in cancer.Nature reviews. Cancer · 2012Review
- Phospholipase D: enzymology, functionality, and chemical modulation.Chemical reviews · 2011Review
- New concepts in phospholipase D signaling in inflammation and cancer.TheScientificWorldJournal · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Phospholipase D (PLD) hydrolyzes phosphatidylcholine to generate phosphatidic acid and choline. Studies in cultured cells and Drosophila melanogaster have implicated PLD in the regulation of many cellular functions, including intracellular vesicle trafficking, cell proliferation and differentiation. However, the function of PLD in vertebrate development has not been explored. Here we report cloning and characterization of a zebrafish PLD1 (pld1) homolog. Like mammalian PLDs, zebrafish Pld1 contains two conservative HKD motifs. Maternally contributed pld1 transcripts are uniformly distributed in early embryo. Localized expression of pld1 is observed in the notochord during early segmentation, in the somites during later segmentation and in the liver at the larval stages. Studies in intact and cell-free preparations demonstrate evolutionary conservation of regulation. Inhibition of Pld1 expression using antisense morpholino oligonucleotides (MO) interfering with the translation or splicing of pld1 impaired intersegmental vessel (ISV) development. Incubating embryos with 1-butanol, which diverts production of phosphatidic acid to a phosphatidylalcohol, caused similar ISV defects. To determine where Pld1 is required for ISV development we performed transplantation experiments. Analyses of the mosaic Pld1 deficient embryos showed partial suppression of ISV defects in the segments containing transplanted wild-type notochord cells but not in the ones containing wild-type somitic cells. These results provide the first evidence that function of Pld1 in the developing notochord is essential for vascular development in vertebrates.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.