ArticleTransfusion2009
Changes in Band 3 oligomeric state precede cell membrane phospholipid loss during blood bank storage of red blood cells.
Article in Transfusion, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Temporal sequence of major biochemical events during blood bank storage of packed red blood cells.Blood transfusion = Trasfusione del sangue · 2012Trial
- Unbiased Morphometric Assessment of Red Blood Cell Storage Lesion in the Presence of Shear-Induced Stomatocytes.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2025Article
- Effects of red blood cell transfusion on patients undergoing cardiac surgery in Queensland - a retrospective cohort study.Journal of cardiothoracic surgery · 2024Article
- Cow blood - A superior storage option in forensics?Heliyon · 2023Article
- Review
- Cyclic Mechanical Stresses Alter Erythrocyte Membrane Composition and Microstructure and Trigger Macrophage Phagocytosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- The interactome of the N-terminus of band 3 regulates red blood cell metabolism and storage quality.Haematologica · 2021Article
- Quantifying dynamic range in red blood cell energetics: Evidence of progressive energy failure during storage.Transfusion · 2021Article
- An Insight into the Stages of Ion Leakage during Red Blood Cell Storage.International journal of molecular sciences · 2021Article
- Two-step process of cytoskeletal structural damage during long-term storage of packed red blood cells.Blood transfusion = Trasfusione del sangue · 2021Article
- Dynamics of shape recovery by stored red blood cells during washing at the single cell level.Transfusion · 2020Article
- Influence of red blood cell-derived microparticles upon vasoregulation.Blood transfusion = Trasfusione del sangue · 2017Review
- Effect of X-ray irradiation on hepatocarcinoma cells and erythrocytes in salvaged blood.Scientific reports · 2017Article
- Physiologic Impact of Circulating RBC Microparticles upon Blood-Vascular Interactions.Frontiers in physiology · 2017Review
- Acid Sphingomyelinase Inhibition Prevents Hemolysis During Erythrocyte Storage.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2016Article
- Molecular mechanisms of erythrocyte aging.Biological chemistry · 2015Review
- The involvement of cation leaks in the storage lesion of red blood cells.Frontiers in physiology · 2014Review
- Red blood cell storage in SAGM and AS3: a comparison through the membrane two-dimensional electrophoresis proteome.Blood transfusion = Trasfusione del sangue · 2012Article
- Red blood cell microparticles and blood group antigens: an analysis by flow cytometry.Blood transfusion = Trasfusione del sangue · 2012Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundLipid loss in the form of vesicles contributes to the red blood cell (RBC) storage lesion, and this loss of lipid is correlated with changes in membrane protein function. Sensitive spectroscopic techniques were used to measure changes in Band 3 oligomeric state during storage of RBCs, compared to metabolic changes and phospholipid loss. The aim of the study was to determine whether changes in the macromolecular organization of membrane proteins occur before, coincident with, or after lipid loss during RBC storage. STUDY DESIGN AND
methodsFive RBC units were collected from normal volunteers and stored under standard blood bank conditions, and both metabolic changes and lipid loss were measured by multiple assays. Band 3 oligomeric state was assessed by time-resolved phosphorescence anisotropy and fluorescence resonance energy transfer of eosin-5-maleimide-labeled RBC ghosts.
resultsExtracellular pH decreased and extracellular potassium increased rapidly during cold storage of blood. Band 3 on the RBC membrane exhibited a shift from small to large oligomers early in the storage period and before detectable loss of phospholipid from the RBC membrane. The immobilized fraction of Band 3, that which is tethered to the cytoskeletal network via spectrin and ankyrin, did not change during cold storage.
conclusionOur results demonstrate that changes in the macromolecular organization of membrane proteins on the RBC occur early in storage, and these changes may induce phospholipid loss, irreversible morphologic changes, and loss of function during RBC storage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.