ArticleJournal of molecular neuroscience : MN2009
Mass spectrometry identifies LGI1-interacting proteins that are involved in synaptic vesicle function in the human brain.
Article in Journal of molecular neuroscience : MN, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- LGI1 acts presynaptically to regulate excitatory synaptic transmission during early postnatal development.Scientific reports · 2016Article
- Homozygous Deletion of the LGI1 Gene in Mice Leads to Developmental Abnormalities Resulting in Cortical Dysplasia.Brain pathology (Zurich, Switzerland) · 2015Article
- Host defense proteins derived from human saliva bind to Staphylococcus aureus.Infection and immunity · 2013Article
- Etiology of a genetically complex seizure disorder in Celf4 mutant mice.Genes, brain, and behavior · 2011Article
- The temporal and spatial expression pattern of the LGI1 epilepsy predisposition gene during mouse embryonic cranial development.BMC neuroscience · 2011Article
- Loss of zebrafish lgi1b leads to hydrocephalus and sensitization to pentylenetetrazol induced seizure-like behavior.PloS one · 2011Article
- Homozygous inactivation of the LGI1 gene results in hypomyelination in the peripheral and central nervous systems.Journal of neuroscience research · 2010Article
- Electroclinical characterization of epileptic seizures in leucine-rich, glioma-inactivated 1-deficient mice.Brain : a journal of neurology · 2010Article
- Investigation of LGI1 as the antigen in limbic encephalitis previously attributed to potassium channels: a case series.The Lancet. Neurology · 2010Article
- Lgi1 null mutant mice exhibit myoclonic seizures and CA1 neuronal hyperexcitability.Human molecular genetics · 2010Article
- Inactivation of LGI1 expression accompanies early stage hyperplasia of prostate epithelium in the TRAMP murine model of prostate cancer.Experimental and molecular pathology · 2010Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The LGI1 gene has been shown to predispose to epilepsy and influence cell invasion in glioma cells. To identify proteins that interact with LGI1 and gain a better understanding of its function, we have used co-immunoprecipitation (co-IP) of a secreted green fluorescent protein-tagged LGI1 protein combined with mass spectrometry to identify interacting partners from lysates prepared from human subcortical white matter. Proteins were recovered from polyacrylamide gels and analyzed using liquid chromatography coupled to tandem mass spectrometry. This analysis identified a range of proteins, but in particular synaptotagmin, synaptophysin, and syntaxin 1A. Each of these proteins is found associated with synaptic vesicles. These interactions were confirmed independently by co-IP and Western blotting and implicate LGI1 in synapse biology in neurons. Other vesicle-related proteins that were recovered by co-IP include clathrin heavy chain 1, syntaxin binding protein 1, and a disintegrin and metalloprotease 23. These observations support a role for LGI1 in synapse vesicle function in neurons.
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