ArticleThe EMBO journal2009
Inter-subunit interaction of gastric H+,K+-ATPase prevents reverse reaction of the transport cycle.
Article in The EMBO journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 89 citations in OpenAlex.
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- Clinical significance of P-class pumps in cancer.Oncology letters · 2021Review
- A single KeLife · 2019Article
- Yeast and human P4-ATPases transport glycosphingolipids using conserved structural motifs.The Journal of biological chemistry · 2019Article
- KScientific reports · 2018Article
- Crystal structures of the gastric proton pump.Nature · 2018Article
- The cryo-EM structure of gastric HScientific reports · 2017Article
- Arginine substitution of a cysteine in transmembrane helix M8 converts Na+,K+-ATPase to an electroneutral pump similar to H+,K+-ATPase.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Biophysical comparison of ATP synthesis mechanisms shows a kinetic advantage for the rotary process.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Computational insights into the interaction mechanism of triazolyl substituted tetrahydrobenzofuran derivatives with H(+),K(+)-ATPase at different pH.Journal of computer-aided molecular design · 2016Article
- Development of the field of structural physiology.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2015Review
- Article
- Systematic comparison of molecular conformations of H+,K+-ATPase reveals an important contribution of the A-M2 linker for the luminal gating.The Journal of biological chemistry · 2014Article
- Article
- Carbon sandwich preparation preserves quality of two-dimensional crystals for cryo-electron microscopy.Microscopy (Oxford, England) · 2013Article
- Article
- Pharmacokinetics and pharmacodynamics of the proton pump inhibitors.Journal of neurogastroenterology and motility · 2013Article
- Influences of membrane mimetic environments on membrane protein structures.Annual review of biophysics · 2013Review
- Active detergent-solubilized H+,K+-ATPase is a monomer.The Journal of biological chemistry · 2012Article
- Cryo-EM structure of gastric H+,K+-ATPase with a single occupied cation-binding site.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The gastric H(+),K(+)-ATPase is an ATP-driven proton pump responsible for generating a million-fold proton gradient across the gastric membrane. We present the structure of gastric H(+),K(+)-ATPase at 6.5 A resolution as determined by electron crystallography of two-dimensional crystals. The structure shows the catalytic alpha-subunit and the non-catalytic beta-subunit in a pseudo-E(2)P conformation. Different from Na(+),K(+)-ATPase, the N-terminal tail of the beta-subunit is in direct contact with the phosphorylation domain of the alpha-subunit. This interaction may hold the phosphorylation domain in place, thus stabilizing the enzyme conformation and preventing the reverse reaction of the transport cycle. Indeed, truncation of the beta-subunit N-terminus allowed the reverse reaction to occur. These results suggest that the beta-subunit N-terminus prevents the reverse reaction from E(2)P to E(1)P, which is likely to be relevant for the generation of a large H(+) gradient in vivo situation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.