ArticleThe EMBO journal2009
S5a promotes protein degradation by blocking synthesis of nondegradable forked ubiquitin chains.
Article in The EMBO journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 78 citations in OpenAlex.
- The role of ubiquitination in health and disease.MedComm · 2024Review
- Proteasome-Bound UCH37/UCHL5 Debranches Ubiquitin Chains to Promote Degradation.Molecular cell · 2020Article
- Release of Ubiquitinated and Non-ubiquitinated Nascent Chains from Stalled Mammalian Ribosomal Complexes by ANKZF1 and Ptrh1.Molecular cell · 2018Article
- K6 linked polyubiquitylation of FADD by CHIP prevents death inducing signaling complex formation suppressing cell death.Oncogene · 2018Article
- Repair or destruction-an intimate liaison between ubiquitin ligases and molecular chaperones in proteostasis.FEBS letters · 2017Review
- The Logic of the 26S Proteasome.Cell · 2017Review
- Ubiquitin Chain Enrichment Middle-Down Mass Spectrometry Enables Characterization of Branched Ubiquitin Chains in Cellulo.Analytical chemistry · 2017Article
- The recognition of ubiquitinated proteins by the proteasome.Cellular and molecular life sciences : CMLS · 2016Review
- Differential Contributions of Ubiquitin-Modified APOBEC3G Lysine Residues to HIV-1 Vif-Induced Degradation.Journal of molecular biology · 2016Article
- Ubiquitin modifications.Cell research · 2016Review
- Identification and characterization of a novel ISG15-ubiquitin mixed chain and its role in regulating protein homeostasis.Scientific reports · 2015Article
- The Proteasome Distinguishes between Heterotypic and Homotypic Lysine-11-Linked Polyubiquitin Chains.Cell reports · 2015Article
- Disassembly of Lys11 and mixed linkage polyubiquitin conjugates provides insights into function of proteasomal deubiquitinases Rpn11 and Ubp6.The Journal of biological chemistry · 2015Article
- Deubiquitinase-based analysis of ubiquitin chain architecture using Ubiquitin Chain Restriction (UbiCRest).Nature protocols · 2015Article
- Angelman syndrome-associated ubiquitin ligase UBE3A/E6AP mutants interfere with the proteolytic activity of the proteasome.Cell death & disease · 2015Article
- Misframed ubiquitin and impaired protein quality control: an early event in Alzheimer's disease.Frontiers in molecular neuroscience · 2015Review
- The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.Oncogene · 2014Article
- MDM2 mediates nonproteolytic polyubiquitylation of the DEAD-Box RNA helicase DDX24.Molecular and cellular biology · 2014Article
- The ubiquitin-conjugating enzyme, UbcM2, is restricted to monoubiquitylation by a two-fold mechanism that involves backside residues of E2 and Lys48 of ubiquitin.Biochemistry · 2014Article
- Degradation of activated K-Ras orthologue via K-Ras-specific lysine residues is required for cytokinesis.The Journal of biological chemistry · 2014Article
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5 authors at 1 institution in 2 countries.
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Abstract
Ubiquitin (Ub)-protein conjugates formed by purified ring-finger or U-box E3s with the E2, UbcH5, resist degradation and disassembly by 26S proteasomes. These chains contain multiple types of Ub forks in which two Ub's are linked to adjacent lysines on the proximal Ub. We tested whether cells contain factors that prevent formation of nondegradable conjugates and whether the forked chains prevent proteasomal degradation. S5a is a ubiquitin interacting motif (UIM) protein present in the cytosol and in the 26S proteasome. Addition of S5a or a GST-fusion of S5a's UIM domains to a ubiquitination reaction containing 26S proteasomes, UbcH5, an E3 (MuRF1 or CHIP), and a protein substrate, dramatically stimulated its degradation, provided S5a was present during ubiquitination. Mass spectrometry showed that S5a and GST-UIM prevented the formation of Ub forks without affecting synthesis of standard isopeptide linkages. The forked Ub chains bind poorly to 26S proteasomes unlike those synthesized with S5a present or linked to Lys63 or Lys48 chains. Thus, S5a (and presumably certain other UIM proteins) function with certain E3/E2 pairs to ensure synthesis of efficiently degraded non-forked Ub conjugates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.