ArticleGene therapy2009
Efficient lentiviral gene transfer into corneal stroma cells using a femtosecond laser.
Article in Gene therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Lentiviral Vectors for Ocular Gene Therapy.Pharmaceutics · 2022Review
- Gene Therapy in the Anterior Eye Segment.Current gene therapy · 2022Review
- Novel insights into gene therapy in the cornea.Experimental eye research · 2021Review
- Corrective GUSB transfer to the canine mucopolysaccharidosis VII cornea using a helper-dependent canine adenovirus vector.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Ocular gene delivery using lentiviral vectors.Gene therapy · 2012Review
- Targeting herpetic keratitis by gene therapy.Journal of ophthalmology · 2012Article
- Corneal transduction by intra-stromal injection of AAV vectors in vivo in the mouse and ex vivo in human explants.PloS one · 2012Article
- Gene therapy in the cornea: 2005--present.Progress in retinal and eye research · 2012Review
- Matrix metalloproteinase 14 overexpression reduces corneal scarring.Gene therapy · 2011Article
- AAV serotype influences gene transfer in corneal stroma in vivo.Experimental eye research · 2010Article
- Strategies for local gene therapy of corneal allograft rejection.Middle East African journal of ophthalmologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated a new procedure for gene transfer into the stroma of pig cornea for the delivery of therapeutic factors. A delimited space was created at 110 mum depth with a LDV femtosecond laser in pig corneas, and a HIV1-derived lentiviral vector expressing green fluorescent protein (GFP) (LV-CMV-GFP) was injected into the pocket. Corneas were subsequently dissected and kept in culture as explants. After 5 days, histological analysis of the explants revealed that the corneal pockets had closed and that the gene transfer procedure was efficient over the whole pocket area. Almost all the keratocytes were transduced in this area. Vector diffusion at right angles to the pocket's plane encompasses four (endothelium side) to 10 (epithelium side) layers of keratocytes. After 21 days, the level of transduction was similar to the results obtained after 5 days. The femtosecond laser technique allows a reliable injection and diffusion of lentiviral vectors to efficiently transduce stromal cells in a delimited area. Showing the efficacy of this procedure in vivo could represent an important step toward treatment or prevention of recurrent angiogenesis of the corneal stroma.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.