ArticleBiology of reproduction2009
Significant improvement in cloning efficiency of an inbred miniature pig by histone deacetylase inhibitor treatment after somatic cell nuclear transfer.
Article in Biology of reproduction, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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Who cites it
105 citing papers in PubMed, 239 citations in OpenAlex.
- Development of a Genetically Engineered Porcine Model of Rhabdoid Tumor Predisposition Syndrome Type 1 (RTPS-1).Cancers · 2026Article
- Epigenetic Drift and the Generational Limit of Serial Somatic Cell Nuclear Transfer in Pigs.Animals : an open access journal from MDPI · 2026Review
- Effect of taxonomical distance and scriptaid on iSCNT embryo development in suidae.Scientific reports · 2026Article
- Pig Genome Editing for Agriculture: Achievements and Challenges.International journal of molecular sciences · 2025Review
- The use of transgenic animals for xenotransplantation: An update.Animal models and experimental medicine · 2025Review
- Scriptaid Improves Cashmere Goat Embryo Reprogramming by Affecting Donor Cell Pluripotency Molecule NANOG Expression.Animals : an open access journal from MDPI · 2025Article
- Trichostatin A-Induced Epigenetic Modifications and Their Influence on the Development of Porcine Cloned Embryos Derived from Bone Marrow-Mesenchymal Stem Cells.International journal of molecular sciences · 2025Article
- Current Techniques of Gene Editing in Pigs for Xenotransplantation.Transplant international : official journal of the European Society for Organ Transplantation · 2025Review
- Effect of Serotonin (5-Hydroxytryptamine) on Follicular Development in Porcine.International journal of molecular sciences · 2024Article
- PigBiobank: a valuable resource for understanding genetic and biological mechanisms of diverse complex traits in pigs.Nucleic acids research · 2024Article
- Myo-inositol improves developmental competence and reduces oxidative stress in porcine parthenogenetic embryos.Frontiers in veterinary science · 2024Article
- Human and Pig Pluripotent Stem Cells: From Cellular Products to Organogenesis and Beyond.Cells · 2023Review
- Mouse somatic cell nuclear transfer: What has changed and remained unchanged in 25 years.The Journal of reproduction and development · 2023Article
- Maslinic Acid Supplementation during the In Vitro Culture Period Ameliorates Early Embryonic Development of Porcine Embryos by Regulating Oxidative Stress.Animals : an open access journal from MDPI · 2023Article
- - Invited Review - Reproductive technologies needed for the generation of precise gene-edited pigs in the pathways from laboratory to farm.Animal bioscience · 2023Article
- Cattle Cloning by Somatic Cell Nuclear Transfer.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Simultaneous Inhibition of Histone Deacetylases and RNA Synthesis Enables Totipotency Reprogramming in Pig SCNT Embryos.International journal of molecular sciences · 2022Article
- Strategies to Improve the Efficiency of Somatic Cell Nuclear Transfer.International journal of molecular sciences · 2022Review
- Enhancement of Chromatin and Epigenetic Reprogramming in Porcine SCNT Embryos-Progresses and Perspectives.Frontiers in cell and developmental biology · 2022Article
- Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species.Development (Cambridge, England) · 2021Article
45 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
The National Institutes of Health (NIH) miniature pig was developed specifically for xenotransplantation and has been extensively used as a large-animal model in many other biomedical experiments. However, the cloning efficiency of this pig is very low (<0.2%), and this has been an obstacle to the promising application of these inbred swine genetics for biomedical research. It has been demonstrated that increased histone acetylation in somatic cell nuclear transfer (SCNT) embryos, by applying a histone deacetylase (HDAC) inhibitor such as trichostatin A (TSA), significantly enhances the developmental competence in several species. However, some researchers also reported that TSA treatment had various detrimental effects on the in vitro and in vivo development of the SCNT embryos. Herein, we report that treatment with 500 nM 6-(1,3-dioxo-1H, 3H-benzo[de]isoquinolin-2-yl)-hexanoic acid hydroxyamide (termed scriptaid), a novel HDAC inhibitor, significantly enhanced the development of SCNT embryos to the blastocyst stage when NIH inbred fetal fibroblast cells (FFCs) were used as donors compared with the untreated group (21% vs. 9%, P < 0.05). Scriptaid treatment resulted in eight pregnancies from 10 embryo transfers (ETs) and 14 healthy NIH miniature pigs from eight litters, while no viable piglets (only three mummies) were obtained from nine ETs in the untreated group. Thus, scriptaid dramatically increased the cloning efficiency when using inbred genetics from 0.0% to 1.3%. In contrast, scriptaid treatment decreased the blastocyst rate in in vitro fertilization embryos (from 37% to 26%, P < 0.05). In conclusion, the extremely low cloning efficiency in the NIH miniature pig may be caused by its inbred genetic background and can be improved by alteration of genomic histone acetylation patterns.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.