ArticleJournal of clinical microbiology2009
Rapid variable-number tandem-repeat genotyping for Mycobacterium leprae clinical specimens.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00315809 (Molecular Epidemiology of Leprosy - Philippines), which is not on this map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Molecular Epidemiology of Leprosy - Philippines
Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- Genetic diversity of Mycobacterium leprae in the state of São Paulo, an area of low-leprosy incidence in Brazil.Revista da Sociedade Brasileira de Medicina Tropical · 2023Article
- Pathogenicity and virulence ofVirulence · 2022Review
- Multiple strain infection of Mycobacterium leprae in a family having 4 patients: A study employing short tandem repeats.PloS one · 2019Article
- Molecular surveillance of antimicrobial resistance and transmission pattern ofEmerging microbes & infections · 2019Article
- Ticks as potential vectors of Mycobacterium leprae: Use of tick cell lines to culture the bacilli and generate transgenic strains.PLoS neglected tropical diseases · 2018Article
- Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region.Pathogens and global health · 2018Article
- Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil.PLoS neglected tropical diseases · 2017Article
- Whole genome sequencing distinguishes between relapse and reinfection in recurrent leprosy cases.PLoS neglected tropical diseases · 2017Article
- Human Genetic Ancestral Composition Correlates with the Origin of Mycobacterium leprae Strains in a Leprosy Endemic Population.PLoS neglected tropical diseases · 2015Article
- A new multiple-locus variable-number tandem repeat analysis reveals different clusters for Anaplasma phagocytophilum circulating in domestic and wild ruminants.Parasites & vectors · 2014Article
- Mycobacterium leprae in Colombia described by SNP7614 in gyrA, two minisatellites and geography.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2013Article
- Molecular, ethno-spatial epidemiology of leprosy in China: novel insights for tracing leprosy in endemic and non endemic provinces.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2013Article
- Detection of dispersed short tandem repeats using reversible jump Markov chain Monte Carlo.Nucleic acids research · 2012Article
- Distribution of Mycobacterium leprae strains among cases in a rural and urban population of Maharashtra, India.Journal of clinical microbiology · 2012Article
- Real-time PCR and high-resolution melt analysis for rapid detection of Mycobacterium leprae drug resistance mutations and strain types.Journal of clinical microbiology · 2012Article
- Transmission of dapsone-resistant leprosy detected by molecular epidemiological approaches.Antimicrobial agents and chemotherapy · 2011Article
- DNA fingerprinting of Mycobacterium leprae strains using variable number tandem repeat (VNTR) - fragment length analysis (FLA).Journal of visualized experiments : JoVE · 2011Article
- Transmission of leprosy in Qiubei County, Yunnan, China: insights from an 8-year molecular epidemiology investigation.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2011Article
- Mycobacterium leprae-host-cell interactions and genetic determinants in leprosy: an overview.Future microbiology · 2011Review
- Mycobacterium leprae: genes, pseudogenes and genetic diversity.Future microbiology · 2011Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Mycobacterium leprae is the noncultivable pathogen of leprosy. Since the genome sequence of an isolate of M. leprae has become available, multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) has been explored as a tool for strain typing and identification of chains of transmission of leprosy. In order to discover VNTRs and develop methods transferable to clinical samples, MLVA was applied to a global collection of M. leprae isolates derived from leprosy patients and propagated in armadillo hosts. PCR amplification, agarose gel electrophoresis, and sequencing methods were applied to DNA extracts from these infected armadillo tissues (n = 21). We identified polymorphisms in 15 out of 25 short-tandem-repeat (STR) loci previously selected by in silico analyses of the M. leprae genome. We then developed multiplex PCR for amplification of these 15 loci in four separate PCRs suitable for fluorescent fragment length analysis and demonstrated STR profiles highly concordant with those from the sequencing methods. Subsequently, we extended this method to DNA extracts from human clinical specimens, such as skin biopsy specimens (n = 30). With these techniques, mapping of multiple loci and differentiation of genotypes have been possible using total DNA extracts from limited amounts of clinical samples at a reduced cost and with less time. These practical methods are therefore available and applicable to answer focused epidemiological questions and to allow monitoring of the transmission of M. leprae in different countries where leprosy is endemic.
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Registered trials
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