ArticleMolecular biology of the cell2009
The mating-specific Galpha interacts with a kinesin-14 and regulates pheromone-induced nuclear migration in budding yeast.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Candida albicans Kinesin Kar3 Depends on a Cik1-Like Regulatory Partner Protein for Its Roles in Mating, Cell Morphogenesis, and Bipolar Spindle Formation.Eukaryotic cell · 2015Article
- Improved Plasmids for Fluorescent Protein Tagging of Microtubules in Saccharomyces cerevisiae.Traffic (Copenhagen, Denmark) · 2015Article
- Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy.Genes & development · 2013Article
- Functional and physical interactions among Saccharomyces cerevisiae α-factor receptors.Eukaryotic cell · 2012Article
- Basidiomycete mating type genes and pheromone signaling.Eukaryotic cell · 2010Review
- Polarization of the yeast pheromone receptor requires its internalization but not actin-dependent secretion.Molecular biology of the cell · 2010Article
- Coordinating mitosis with cell polarity: Molecular motors at the cell cortex.Seminars in cell & developmental biology · 2010Review
- When yeast cells meet, karyogamy!: an example of nuclear migration slowly resolved.Nucleus (Austin, Tex.)Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
As a budding yeast cell elongates toward its mating partner, cytoplasmic microtubules connect the nucleus to the cell cortex at the growth tip. The Kar3 kinesin-like motor protein is then thought to stimulate plus-end depolymerization of these microtubules, thus drawing the nucleus closer to the site where cell fusion and karyogamy will occur. Here, we show that pheromone stimulates a microtubule-independent interaction between Kar3 and the mating-specific Galpha protein Gpa1 and that Gpa1 affects both microtubule orientation and cortical contact. The membrane localization of Gpa1 was found to polarize early in the mating response, at about the same time that the microtubules begin to attach to the incipient growth site. In the absence of Gpa1, microtubules lose contact with the cortex upon shrinking and Kar3 is improperly localized, suggesting that Gpa1 is a cortical anchor for Kar3. We infer that Gpa1 serves as a positional determinant for Kar3-bound microtubule plus ends during mating.
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