ArticleThe Journal of biological chemistry2009
The action of 11-cis-retinol on cone opsins and intact cone photoreceptors.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- Retinoid dynamics in vision: from visual cycle biology to retina disease treatments.Pharmacology & therapeutics · 2025Review
- Chromophore supply modulates cone function and survival in retinitis pigmentosa mouse models.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Rods progressively escape saturation to drive visual responses in daylight conditions.Nature communications · 2017Article
- The role of retinol dehydrogenase 10 in the cone visual cycle.Scientific reports · 2017Article
- The retina visual cycle is driven by cis retinol oxidation in the outer segments of cones.Visual neuroscience · 2017Article
- cis Retinol oxidation regulates photoreceptor access to the retina visual cycle and cone pigment regeneration.The Journal of physiology · 2016Article
- Retinol dehydrogenase 8 and ATP-binding cassette transporter 4 modulate dark adaptation of M-cones in mammalian retina.The Journal of physiology · 2015Article
- Retinal Attachment Instability Is Diversified among Mammalian Melanopsins.The Journal of biological chemistry · 2015Article
- Dephosphorylation during bleach and regeneration of visual pigment in carp rod and cone membranes.The Journal of biological chemistry · 2015Article
- RDH13L, an enzyme responsible for the aldehyde-alcohol redox coupling reaction (AL-OL coupling reaction) to supply 11-cis retinal in the carp cone retinoid cycle.The Journal of biological chemistry · 2015Article
- High-throughput RNA sequencing reveals the effects of 2,2',4,4' -tetrabromodiphenyl ether on retina and bone development of zebrafish larvae.BMC genomics · 2015Article
- Rhodopsin in the rod surface membrane regenerates more rapidly than bulk rhodopsin in the disc membranes in vivo.The Journal of physiology · 2014Article
- Substrate specificity and subcellular localization of the aldehyde-alcohol redox-coupling reaction in carp cones.The Journal of biological chemistry · 2013Article
- New insights into retinoid metabolism and cycling within the retina.Progress in retinal and eye research · 2013Review
- Explant cultures of Rpe65-/- mouse retina: a model to investigate cone opsin trafficking.Molecular vision · 2013Article
- Gap junctions are essential for generating the correlated spike activity of neighboring retinal ganglion cells.PloS one · 2013Article
- RPE65 is present in human green/red cones and promotes photopigment regeneration in an in vitro cone cell model.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Vitamin A activates rhodopsin and sensitizes it to ultraviolet light.Visual neuroscience · 2011Article
- Regeneration of photopigment is enhanced in mouse cone photoreceptors expressing RPE65 protein.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Probing human red cone opsin activity with retinal analogues.Journal of natural products · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
11-cis-retinol has previously been shown in physiological experiments to promote dark adaptation and recovery of photoresponsiveness of bleached salamander red cones but not of bleached salamander red rods. The purpose of this study was to evaluate the direct interaction of 11-cis-retinol with expressed human and salamander cone opsins, and to determine by microspectrophotometry pigment formation in isolated salamander photoreceptors. We show here in a cell-free system using incorporation of radioactive guanosine 5'-3-O-(thio)triphosphate into transducin as an index of activity, that 11-cis-retinol inactivates expressed salamander cone opsins, acting an inverse agonist. Similar results were obtained with expressed human red and green opsins. 11-cis-retinol had no significant effect on the activity of human blue cone opsin. In contrast, 11-cis-retinol activates the expressed salamander and human red rod opsins, acting as an agonist. Using microspectrophotometry of salamander cone photoreceptors before and after bleaching and following subsequent treatment with 11-cis-retinol, we show that 11-cis-retinol promotes pigment formation. Pigment was not formed in salamander red rods or green rods (containing the same opsin as blue cones) treated under the same conditions. These results demonstrate that 11-cis-retinol is not a useful substrate for rod photoreceptors although it is for cone photoreceptors. These data support the premise that rods and cones have mechanisms for handling retinoids and regenerating visual pigment that are specific to photoreceptor type. These mechanisms are critical to providing regenerated pigments in a time scale required for the function of these two types of photoreceptors.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.