ArticlePloS one2009
The ordered extension of pseudopodia by amoeboid cells in the absence of external cues.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed.
- Reply to van Haastert: Local competition between Ras/actin-driven protrusions.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Local Ras/actin signaling orients individual pseudopods in shallow gradients.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Excitable Ras dynamics-based screens reveal RasGEFX is required for macropinocytosis and random cell migration.Nature communications · 2025Article
- Approximate Bayesian inference in a model for self-generated gradient collective cell movement.Computational statistics · 2025Article
- Dynamic cluster field modeling of collective chemotaxis.Scientific reports · 2024Article
- Pseudopod Tracking and Statistics During Cell Movement in Buffer and Chemotaxis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Three-component contour dynamics model to simulate and analyze amoeboid cell motility in two dimensions.PloS one · 2024Article
- Active oscillations in microscale navigation.Animal cognition · 2023Review
- Orientation of Cell Polarity by Chemical Gradients.Annual review of biophysics · 2022Review
- Mechanochemical subcellular-element model of crawling cells.Frontiers in cell and developmental biology · 2022Article
- Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity.Biophysics and physicobiology · 2022Article
- Cellular inertia.Scientific reports · 2021Article
- Coupling traction force patterns and actomyosin wave dynamics reveals mechanics of cell motion.Molecular systems biology · 2021Article
- Comparative mapping of crawling-cell morphodynamics in deep learning-based feature space.PLoS computational biology · 2021Article
- Analysis of protrusion dynamics in amoeboid cell motility by means of regularized contour flows.PLoS computational biology · 2021Article
- Adhesion strength and contractility enable metastatic cells to become adurotactic.Cell reports · 2021Article
- Two Complementary Signaling Pathways Depict Eukaryotic Chemotaxis: A Mechanochemical Coupling Model.Frontiers in cell and developmental biology · 2021Article
- Short- and long-term memory of moving amoeboid cells.PloS one · 2021Article
- Tools for computational analysis of moving boundary problems in cellular mechanobiology.Wiley interdisciplinary reviews. Systems biology and medicine · 2020Review
- Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic cells extend pseudopodia for movement. In the absence of external cues, cells move in random directions, but with a strong element of persistence that keeps them moving in the same direction Persistence allows cells to disperse over larger areas and is instrumental to enter new environments where spatial cues can lead the cell. Here we explore cell movement by analyzing the direction, size and timing of approximately 2000 pseudopodia that are extended by Dictyostelium cells. The results show that pseudpopod are extended perpendicular to the surface curvature at the place where they emerge. The location of new pseudopods is not random but highly ordered. Two types of pseudopodia may be formed: frequent splitting of an existing pseudopod, or the occasional extension of a de novo pseudopod at regions devoid of recent pseudopod activity. Split-pseudopodia are extended at approximately 60 degrees relative to the previous pseudopod, mostly as alternating Right/Left/Right steps leading to relatively straight zigzag runs. De novo pseudopodia are extended in nearly random directions thereby interrupting the zigzag runs. Persistence of cell movement is based on the ratio of split versus de novo pseudopodia. We identify PLA2 and cGMP signaling pathways that modulate this ratio of splitting and de novo pseudopodia, and thereby regulate the dispersal of cells. The observed ordered extension of pseudopodia in the absence of external cues provides a fundamental insight into the coordinated movement of cells, and might form the basis for movement that is directed by internal or external cues.
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