ArticleBiophysical journal2009
Cardiac myosin binding protein-C is essential for thick-filament stability and flexural rigidity.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Design Principles and Benefits of Spatially Explicit Models of Myofilament Function.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Interacting-heads motif explains the X-ray diffraction pattern of relaxed vertebrate skeletal muscle.Biophysical journal · 2022Article
- Geometric basis of action potential of skeletal muscle cells and neurons.Open life sciences · 2022Review
- Contiguity and Structural Impacts of a Non-Myosin Protein within the Thick Filament Myosin Layers.Biology · 2021Article
- Secondary Structure of the Novel Myosin Binding Domain WYR and Implications within Myosin Structure.Biology · 2021Article
- Cardiac myosin binding protein-C phosphorylation accelerates β-cardiac myosin detachment rate in mouse myocardium.American journal of physiology. Heart and circulatory physiology · 2021Article
- A feline orthologue of the human MYH7 c.5647G>A (p.(Glu1883Lys)) variant causes hypertrophic cardiomyopathy in a Domestic Shorthair cat.European journal of human genetics : EJHG · 2019Article
- Article
- Contractile apparatus dysfunction early in the pathophysiology of diabetic cardiomyopathy.World journal of diabetes · 2015Review
- Kinetics of cardiac myosin isoforms in mouse myocardium are affected differently by presence of myosin binding protein-C.Journal of muscle research and cell motility · 2014Article
- Molecular modulation of actomyosin function by cardiac myosin-binding protein C.Pflugers Archiv : European journal of physiology · 2014Review
- An evolutionary analysis of flightin reveals a conserved motif unique and widespread in Pancrustacea.Journal of molecular evolution · 2014Article
- Impaired contractile function due to decreased cardiac myosin binding protein C content in the sarcomere.American journal of physiology. Heart and circulatory physiology · 2013Article
- Myosin heads are displaced from actin filaments in the in situ beating rat heart in early diabetes.Biophysical journal · 2013Article
- A two-segment model for thin filament architecture in skeletal muscle.Nature reviews. Molecular cell biology · 2013Article
- In vivo cardiac myosin binding protein C gene transfer rescues myofilament contractile dysfunction in cardiac myosin binding protein C null mice.Circulation. Heart failure · 2012Article
- Structure, interactions and function of the N-terminus of cardiac myosin binding protein C (MyBP-C): who does what, with what, and to whom?Journal of muscle research and cell motility · 2012Review
- Roles for cardiac MyBP-C in maintaining myofilament lattice rigidity and prolonging myosin cross-bridge lifetime.Biophysical journal · 2011Article
- Protein kinase A-induced myofilament desensitization to Ca(2+) as a result of phosphorylation of cardiac myosin-binding protein C.The Journal of general physiology · 2010Article
- Phosphorylation and function of cardiac myosin binding protein-C in health and disease.Journal of molecular and cellular cardiology · 2010Review
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Using atomic force microscopy, we examined the contribution of cardiac myosin binding protein-C (cMyBP-C) to thick-filament length and flexural rigidity. Native thick filaments were isolated from the hearts of transgenic mice bearing a truncation mutation of cMyBP-C (t/t) that results in no detectable cMyBP-C and from age-matched wild-type controls (+/+). Atomic force microscopy images of these filaments were evaluated with an automated analysis algorithm that identified filament position and shape. The t/t thick-filament length (1.48 +/- 0.02 microm) was significantly (P < 0.01) shorter than +/+ (1.56 +/- 0.02 microm). This 5%-shorter thick-filament length in the t/t was reflected in 4% significantly shorter sarcomere lengths of relaxed isolated cardiomyocytes of the t/t (1.97 +/- 0.01 microm) compared to +/+ (2.05 +/- 0.01 microm). To determine if cMyBP-C contributes to the mechanical properties of thick filaments, we used statistical polymer chain mechanics to calculate a per-filament-specific persistence length, an index of flexural rigidity directly proportional to Young's modulus. Thick-filament-specific persistence length in the t/t (373 +/- 62 microm) was significantly lower than in +/+ (639 +/- 101 microm). Accordingly, Young's modulus of t/t thick filaments was approximately 60% of +/+. These results provide what we consider a new understanding for the critical role of cMyBP-C in defining normal cardiac output by sustaining force and muscle stiffness.
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Registered trials
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