ArticleBiophysical journal2009
Electrostatic regulation of genome packaging in human hepatitis B virus.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Packaging contests between viral RNA molecules and kinetic selectivity.PLoS computational biology · 2022Article
- Pressurized DNA state inside herpes capsids-A novel antiviral target.PLoS pathogens · 2020Article
- Cation-Anion Interactions within the Nucleic Acid Ion Atmosphere Revealed by Ion Counting.Journal of the American Chemical Society · 2015Article
- A Thermodynamic Model for Genome Packaging in Hepatitis B Virus.Biophysical journal · 2015Article
- The interface between hepatitis B virus capsid proteins affects self-assembly, pregenomic RNA packaging, and reverse transcription.Journal of virology · 2015Article
- A theoretical study of SRPK interaction with the flexible domains of hepatitis B capsids.Biophysical journal · 2014Article
- Pathways for virus assembly around nucleic acids.Journal of molecular biology · 2014Article
- Encapsidated hepatitis B virus reverse transcriptase is poised on an ordered RNA lattice.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Understanding nucleic acid-ion interactions.Annual review of biochemistry · 2014Review
- Density functional theory for encapsidated polyelectrolytes: a comparison with Monte Carlo simulation.The Journal of chemical physics · 2012Article
- A theoretical model for the dynamic structure of hepatitis B nucleocapsid.Biophysical journal · 2011Article
- Thermodynamic basis for the genome to capsid charge relationship in viral encapsidation.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Serine phosphoacceptor sites within the core protein of hepatitis B virus contribute to genome replication pleiotropically.PloS one · 2011Article
- The arginine clusters of the carboxy-terminal domain of the core protein of hepatitis B virus make pleiotropic contributions to genome replication.Journal of virology · 2011Article
- Encapsulation of a polymer by an icosahedral virus.Physical biology · 2010Article
- Mechanisms of capsid assembly around a polymer.Biophysical journal · 2010Article
- Full-length hepatitis B virus core protein packages viral and heterologous RNA with similarly high levels of cooperativity.Journal of virology · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hepatitis B virus (HBV) is a contagious human pathogen causing liver diseases such as cirrhosis and hepatocellular carcinoma. An essential step during HBV replication is packaging of a pregenomic (pg) RNA within the capsid of core antigens (HBcAgs) that each contains a flexible C-terminal tail rich in arginine residues. Mutagenesis experiments suggest that pgRNA encapsidation hinges on its strong electrostatic interaction with oppositely charged C-terminal tails of the HBcAgs, and that the net charge of the capsid and C-terminal tails determines the genome size and nucleocapsid stability. Here, we elucidate the biophysical basis for electrostatic regulation of pgRNA packaging in HBV by using a coarse-grained molecular model that explicitly accounts for all nonspecific interactions among key components within the nucleocapsid. We find that for mutants with variant C-terminal length, an optimal genome size minimizes an appropriately defined thermodynamic free energy. The thermodynamic driving force of RNA packaging arises from a combination of electrostatic interactions and molecular excluded-volume effects. The theoretical predictions of the RNA length and nucleocapsid internal structure are in good agreement with available experiments for the wild-type HBV and mutants with truncated HBcAg C-termini.
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