ArticleTissue engineering. Part C, Methods2010
Dynamic 3D culture promotes spontaneous embryonic stem cell differentiation in vitro.
Article in Tissue engineering. Part C, Methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed.
- Evaluating Strategies to Assess the Differentiation Potential of Human Pluripotent Stem Cells: A Review, Analysis and Call for Innovation.Stem cell reviews and reports · 2025Review
- Article
- Applications of novel bioreactor technology to enhance the viability and function of cultured cells and tissues.Interface focus · 2020Article
- Human Organ-Specific 3D Cancer Models Produced by the Stromal Self-Assembly Method of Tissue Engineering for the Study of Solid Tumors.BioMed research international · 2020Review
- Engineering Strategies for the Formation of Embryoid Bodies from Human Pluripotent Stem Cells.Stem cells and development · 2015Review
- Induction of Hepatic and Endothelial Differentiation by Perfusion in a Three-Dimensional Cell Culture Model of Human Fetal Liver.Tissue engineering. Part C, Methods · 2015Article
- Analysis of type II diabetes mellitus adipose-derived stem cells for tissue engineering applications.Journal of tissue engineering · 2015Article
- Formation of well-defined embryoid bodies from dissociated human induced pluripotent stem cells using microfabricated cell-repellent microwell arrays.Scientific reports · 2014Article
- Bioreactors addressing diabetes mellitus.Journal of diabetes science and technology · 2014Review
- Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor.Stem cells and development · 2013Article
- Several important in vitro improvements in the amplification, differentiation and tracing of fetal liver stem/progenitor cells.PloS one · 2012Article
- Erythrocyte enrichment in hematopoietic progenitor cell cultures based on magnetic susceptibility of the hemoglobin.PloS one · 2012Article
- Optimization of a serum-free culture medium for mouse embryonic stem cells using design of experiments (DoE) methodology.Cytotechnology · 2010Article
- Embryoid body formation from embryonic and induced pluripotent stem cells: Benefits of bioreactors.World journal of stem cells · 2009Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Spontaneous in vitro differentiation of mouse embryonic stem cells (mESC) is promoted by a dynamic, three-dimensional (3D), tissue-density perfusion technique with continuous medium perfusion and exchange in a novel four-compartment, interwoven capillary bioreactor. We compared ectodermal, endodermal, and mesodermal immunoreactive tissue structures formed by mESC at culture day 10 with mouse fetal tissue development at gestational day E9.5. The results show that the bioreactor cultures more closely resemble mouse fetal tissue development at gestational day E9.5 than control mESC cultured in Petri dishes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.