ArticlePhotosynthesis research2009
A simple colorimetric determination of the manganese content in photosynthetic membranes.
Article in Photosynthesis research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- High-efficiency oxygen evolution by photosystem II oxygen-evolving complex containing 3Mn per reaction center.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2023Article
- Competitive interaction of Mn(II) and Fe(II) cations with the high-affinity Mn-binding site of the photosystem II: evolutionary aspect.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2022Article
- Effective binding of TbJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2021Article
- CaPhotosynthesis research · 2021Article
- Photovoltage generation by photosystem II core complexes immobilized onto a Millipore filter on an indium tin oxide electrode.Journal of bioenergetics and biomembranes · 2020Article
- pH dependence of photosystem II photoinhibition: relationship with structural transition of oxygen-evolving complex at the pH of thylakoid lumen.Photosynthesis research · 2020Article
- Substituting Fe for two of the four Mn ions in photosystem II-effects on water-oxidation.Journal of bioenergetics and biomembranes · 2016Article
- Correlation between pH dependence of O2 evolution and sensitivity of Mn cations in the oxygen-evolving complex to exogenous reductants.Photosynthesis research · 2015Article
- The extrinsic PsbO protein modulates the oxidation/reduction rate of the exogenous Mn cation at the high-affinity Mn-binding site of Mn-depleted PSII membranes.Journal of bioenergetics and biomembranes · 2015Article
- Production of reactive oxygen species in decoupled, Ca(2+)-depleted PSII and their use in assigning a function to chloride on both sides of PSII.Photosynthesis research · 2013Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The functional Mn content of intact photosystem II membrane fragments was measured as 4.06 +/- 0.13 Mn/reaction center when determined using a simple, sensitive colorimetric assay that will also work with thylakoids and core complexes. This procedure requires minimal sample material, does not need expensive assay equipment, requires four simple steps, and only takes 20-30 min to perform. These include (a) removal of the adventitious Mn ions by CaCl(2) treatment of the membranes, (b) extraction of the Mn from the O(2)-evolving complex with hydrochloric acid, (c) purification of the extract by centrifugation followed by filtration of the supernatant through an Acrodisc syringe filter (0.2 mum nylon membrane), and (d) colorimetric determination of Mn in the extract using the reaction of the chromogenic agent, 3,3',5,5'-tetramethylbenzidine, with previously oxidized Mn(II) cations carried out at high pH. The colorimetric assay itself has been used previously by Serrat (Mikrochim Acta 129:77-80, 1998) for assaying Mn concentrations in sea water and drinking water.
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