ArticlePloS one2009
Freeze-drying of mononuclear cells derived from umbilical cord blood followed by colony formation.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Stallion Sperm Lyophilisation: Effect of Source of Sperm and Reproductive Season.Veterinary sciences · 2026Article
- Evaluating bacterial growth in raw, frozen, and heat-treated colostrum inoculated with fecalJDS communications · 2025Article
- Functional characterization and therapeutic potential of human umbilical cord blood mononuclear cells.Regenerative therapy · 2025Review
- Low-temperature vacuum evaporation as a novel dehydration process for the long-term preservation of transplantable human corneal tissue.Cell and tissue banking · 2025Article
- Efficacy of stem cell secretome loaded in hyaluronate sponge for topical treatment of psoriasis.Bioengineering & translational medicine · 2023Article
- Healthy cloned offspring derived from freeze-dried somatic cells.Nature communications · 2022Article
- Human Placental Mesenchymal Stem Cells for the Treatment of ARDS in Rat.Stem cells international · 2022Article
- Development and Application of a Mechanistic Cooling and Freezing Model of the Spin Freezing Step within the Framework of Continuous Freeze-Drying.Pharmaceutics · 2021Article
- Cryopreservation of NK and T Cells Without DMSO for Adoptive Cell-Based Immunotherapy.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Review
- Lyopreserved amniotic membrane is cellularly and clinically similar to cryopreserved construct for treating foot ulcers.International wound journal · 2020Article
- An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages.Scientific reports · 2018Article
- Lyophilized human cells stored at room temperature preserve multiple RNA species at excellent quality for RNA sequencing.Oncotarget · 2018Article
- High post-thaw survival of ram sperm after partial freeze-drying.Journal of assisted reproduction and genetics · 2018Article
- Properties of viable lyopreserved amnion are equivalent to viable cryopreserved amnion with the convenience of ambient storage.PloS one · 2018Article
- Article
- Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze-dried bone marrow stem cell paracrine factors.Oncology letters · 2017Article
- Article
- Lyophilized platelet-rich fibrin (PRF) promotes craniofacial bone regeneration through Runx2.International journal of molecular sciences · 2014Article
- The impact of biosampling procedures on molecular data interpretation.Molecular & cellular proteomics : MCP · 2013Review
- Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe recently showed that freeze-dried cells stored for 3 years at room temperature can direct embryonic development following cloning. However, viability, as evaluated by membrane integrity of the cells after freeze-drying, was very low; and it was mainly the DNA integrity that was preserved. In the present study, we improved the cells' viability and functionality after freeze-drying. METHODOLOGY/PRINCIPAL
findingsWe optimized the conditions of directional freezing, i.e. interface velocity and cell concentration, and we added the antioxidant EGCG to the freezing solution. The study was performed on mononuclear cells (MNCs) derived from human umbilical cord blood. After freeze-drying, we tested the viability, number of CD34(+)-presenting cells and ability of the rehydrated hematopoietic stem cells to differentiate into different blood cells in culture. The viability of the MNCs after freeze-drying and rehydration with pure water was 88%-91%. The total number of CD34(+)-presenting cells and the number of colonies did not change significantly when evaluated before freezing, after freeze-thawing, and after freeze-drying (5.4 x 10(4)+/-4.7, 3.49 x 10(4)+/-6 and 6.31 x 10(4)+/-12.27 cells, respectively, and 31+/-25.15, 47+/-45.8 and 23.44+/-13.3 colonies, respectively).
conclusionsThis is the first report of nucleated cells which have been dried and then rehydrated with double-distilled water remaining viable, and of hematopoietic stem cells retaining their ability to differentiate into different blood cells.
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