ArticleThe Journal of experimental medicine2009
The activation-induced cytidine deaminase (AID) efficiently targets DNA in nucleosomes but only during transcription.
Article in The Journal of experimental medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 60 citations in OpenAlex.
- DNA secondary structures in BCL2 and MYC elicit activation-induced cytidine deaminase binding and activity.Nucleic acids research · 2026Article
- Engineering and optimising deaminase fusions for genome editing.Nature communications · 2016Article
- Effects of prostaglandin E2 on p53 mRNA transcription and p53 mutagenesis during T-cell-independent human B-cell clonal expansion.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- AID and Apobec3G haphazard deamination and mutational diversity.Cellular and molecular life sciences : CMLS · 2013Review
- The AID-induced DNA damage response in chromatin.Molecular cell · 2013Review
- Regulation of Aicda expression and AID activity.Autoimmunity · 2013Review
- Multiple clonal MLL fusions in a patient receiving CHOP-based chemotherapy.British journal of haematology · 2012Article
- Immunoglobulin class-switch DNA recombination: induction, targeting and beyond.Nature reviews. Immunology · 2012Review
- Nucleosome stability dramatically impacts the targeting of somatic hypermutation.Molecular and cellular biology · 2012Article
- APOBEC3 proteins and genomic stability: the high cost of a good defense.Cell cycle (Georgetown, Tex.) · 2012Article
- The DSIF subunits Spt4 and Spt5 have distinct roles at various phases of immunoglobulin class switch recombination.PLoS genetics · 2012Article
- Evolution of coordinated mutagenesis and somatic hypermutation in VH5.Molecular immunology · 2011Article
- Epigenetic reprogramming: is deamination key to active DNA demethylation?Reproduction (Cambridge, England) · 2011Review
- AID: a riddle wrapped in a mystery inside an enigma.Immunologic research · 2011Review
- Targets of somatic hypermutation within immunoglobulin light chain genes in zebrafish.Immunology · 2011Article
- Article
- High resolution analysis of the chromatin landscape of the IgE switch region in human B cells.PloS one · 2011Article
- Histone3 lysine4 trimethylation regulated by the facilitates chromatin transcription complex is critical for DNA cleavage in class switch recombination.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Cytidine deaminases: AIDing DNA demethylation?Genes & development · 2010Review
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The activation-induced cytidine deaminase (AID) initiates somatic hypermutation, class-switch recombination, and gene conversion of immunoglobulin genes. In vitro, AID has been shown to target single-stranded DNA, relaxed double-stranded DNA, when transcribed, or supercoiled DNA. To simulate the in vivo situation more closely, we have introduced two copies of a nucleosome positioning sequence, MP2, into a supercoiled AID target plasmid to determine where around the positioned nucleosomes (in the vicinity of an ampicillin resistance gene) cytidine deaminations occur in the absence or presence of transcription. We found that without transcription nucleosomes prevented cytidine deamination by AID. However, with transcription AID readily accessed DNA in nucleosomes on both DNA strands. The experiments also showed that AID targeting any DNA molecule was the limiting step, and they support the conclusion that once targeted to DNA, AID acts processively in naked DNA and DNA organized within transcribed nucleosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.