ArticleAntimicrobial agents and chemotherapy2009
Genetic and biochemical characterization of the first extended-spectrum CARB-type beta-lactamase, RTG-4, from Acinetobacter baumannii.
Article in Antimicrobial agents and chemotherapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 51 citations in OpenAlex.
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- Prevalence and virulence of Vibrio parahaemolyticus isolated from clinical and environmental samples in Huzhou, China.BMC genomics · 2024Article
- CRISPR-Based Gene Editing inPharmaceuticals (Basel, Switzerland) · 2023Review
- Prevalence and antimicrobial resistance profiles ofFrontiers in microbiology · 2023Article
- Detection of expanded-spectrum cephalosporin hydrolysis by lateral flow immunoassay.Microbial biotechnology · 2022Article
- Comparison of Three Expanded-Spectrum Cephalosporin Hydrolysis Assays and the NG-Test CTX-M Multi Assay That Detects All CTX-M-Like Enzymes.Diagnostics (Basel, Switzerland) · 2022Article
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- Multidrug-ResistantLife (Basel, Switzerland) · 2021Article
- Extended Spectrum Beta-Lactamase (ESBL) Produced by Gram-Negative Bacteria in Trinidad and Tobago.International journal of microbiology · 2021Review
- Antibiotic Resistance Profiles, Molecular Mechanisms and Innovative Treatment Strategies ofMicroorganisms · 2020Review
- ISMobile DNA · 2020Review
- Biology ofFrontiers in cellular and infection microbiology · 2017Review
- Quantitative assessment of insertion sequence impact on bacterial genome architecture.Microbial genomics · 2016Article
- A Structure-Based Classification of Class A β-Lactamases, a Broadly Diverse Family of Enzymes.Clinical microbiology reviews · 2016Review
- Acinetobacter calcoaceticus from a fatal case of pneumonia harboring bla(NDM-1) on a widely distributed plasmid.BMC infectious diseases · 2015Article
- CARB-17 family of β-lactamases mediates intrinsic resistance to penicillins in Vibrio parahaemolyticus.Antimicrobial agents and chemotherapy · 2015Article
- Antimicrobial resistance in Acinetobacter baumannii: From bench to bedside.World journal of clinical cases · 2014Review
- The Acinetobacter baumannii Oxymoron: Commensal Hospital Dweller Turned Pan-Drug-Resistant Menace.Frontiers in microbiology · 2012Article
- Emergence of metallo-β-lactamase NDM-1-producing multidrug-resistant Escherichia coli in Australia.Antimicrobial agents and chemotherapy · 2010Article
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Authors and funding
5 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acinetobacter baumannii isolate KAR was uncommonly more resistant to cefepime and cefpirome than to ceftazidime and cefotaxime. Cloning and expression of the beta-lactamase gene content of this isolate into Escherichia coli TOP10 identified ss-lactamase RTG-4 (or CARB-10), which corresponds to the first reported extended-spectrum CARB-type enzyme. RTG-4 is a plasmid-encoded Ambler class A beta-lactamase whose sequence differs by 4 amino acid substitutions from the narrow-spectrum beta-lactamase RTG-3. RTG-4 hydrolyzes cefepime and cefpirome and weakly hydrolyzes ceftazidime due to the single Ser-to-Thr substitution at Ambler position 69. RTG-4 is less susceptible to inhibition by tazobactam and sulbactam than RTG-3. Expression of beta-lactamase RTG-4 in a wild-type A. baumannii reference strain showed that it conferred resistance to cefepime and cefpirome. The genetic environment of the bla(RTG-4) gene was made of a peculiar transposon located on a ca. 50-kb plasmid. ISAba9, located upstream of bla(RTG-4), may be responsible for its acquisition by recognizing a secondary right inverted repeat sequence, thus acting by a one-ended transposition process.
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