ArticleMolecular and cellular biology2009
Locally, meiotic double-strand breaks targeted by Gal4BD-Spo11 occur at discrete sites with a sequence preference.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Spo11: from topoisomerase VI to meiotic recombination initiator.Biochemical Society transactions · 2025Review
- dCas9-SPO11-1 locally stimulates meiotic recombination in rice.Frontiers in plant science · 2025Article
- Reconstitution of SPO11-dependent double-strand break formation.bioRxiv : the preprint server for biology · 2024Article
- Fungal resilience and host-pathogen interactions: Future perspectives and opportunities.Parasite immunology · 2023Review
- Review
- Article
- Structural and functional characterization of the Spo11 core complex.Nature structural & molecular biology · 2021Article
- Mechanism and Control of Meiotic DNA Double-Strand Break Formation inFrontiers in cell and developmental biology · 2021Review
- Mating-type switching by homology-directed recombinational repair: a matter of choice.Current genetics · 2019Review
- Programming sites of meiotic crossovers using Spo11 fusion proteins.Nucleic acids research · 2017Article
- Genomic and chromatin features shaping meiotic double-strand break formation and repair in mice.Cell cycle (Georgetown, Tex.) · 2017Article
- A global view of meiotic double-strand break end resection.Science (New York, N.Y.) · 2017Article
- Article
- Review
- Meiotic DSB patterning: A multifaceted process.Cell cycle (Georgetown, Tex.) · 2016Review
- GC-biased gene conversion in yeast is specifically associated with crossovers: molecular mechanisms and evolutionary significance.Molecular biology and evolution · 2013Article
- Article
- Meiosis: making a break for it.Current opinion in cell biology · 2010Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Meiotic recombination is initiated by DNA double-strand breaks (DSBs) that are catalyzed by the type II topoisomerase-like Spo11 protein. Locally, at recombination hot spots, Spo11 introduces DSBs at multiple positions within approximately 75 to 250 bp, corresponding to accessible regions of the chromatin. The molecular basis of this multiplicity of cleavage positions, observed in a population of meiotic cells, remains elusive. To address this issue, we have examined the properties of the Gal4BD-Spo11 fusion protein, which targets meiotic DSBs to regions with Gal4 binding sites (UAS). By single-nucleotide resolution mapping of targeted DSBs, we found that DSB formation was restricted to discrete sites approximately 20 nucleotides from the UAS, defining a "DSB targeting window." Thus, the multiplicity of cleavage positions at natural Spo11 hot spots likely represents binding of Spo11 to different distinct sites within the accessible DNA region in each different meiotic cell. Further, we showed that mutations in the Spo11 moiety affected the DSB distribution in the DSB targeting window and that mutations in the DNA at the Spo11 cleavage site affected DSB position. These results demonstrate that Spo11 itself has sequence preference and contributes to the choice of DSB positions.
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