ArticleMolecular and cellular biology2009
Genome-wide mapping of boundary element-associated factor (BEAF) binding sites in Drosophila melanogaster links BEAF to transcription.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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76 citing papers in PubMed, 110 citations in OpenAlex.
- Dosage compensation defects due to roX RNA loss are rescued by recalibration of X/autosome stoichiometry.Nucleic acids research · 2026Article
- TheGenes · 2026Article
- Characterization of core promoter activation by the Drosophila insulator-binding protein BEAF.Scientific reports · 2025Article
- Insulator BEAF32 regulates expression of tissue-specific genes and piRNA source loci in Drosophila ovaries.Epigenetics & chromatin · 2025Article
- The homie insulator has sub-elements with different insulating and long-range pairing properties.Genetics · 2025Article
- ThebioRxiv : the preprint server for biology · 2025Article
- L2G: Repurposing Language Models for Genomics Tasks.bioRxiv : the preprint server for biology · 2024Article
- Su(Hw) interacts with Combgap to establish long-range chromatin contacts.Epigenetics & chromatin · 2024Article
- Enhancer-promoter interactions become more instructive in the transition from cell-fate specification to tissue differentiation.Nature genetics · 2024Article
- 3D chromatin interactions involving Drosophila insulators are infrequent but preferential and arise before TADs and transcription.Nature communications · 2023Article
- CTCF, BEAF-32, and CP190 are not required for the establishment of TADs in earlyScience advances · 2023Article
- The chromatin factor ROW cooperates with BEAF-32 in regulating long-range inducible genes.EMBO reports · 2022Article
- The Drosophila BEAF insulator protein interacts with the polybromo subunit of the PBAP chromatin remodeling complex.G3 (Bethesda, Md.) · 2022Article
- Functions of HP1 proteins in transcriptional regulation.Epigenetics & chromatin · 2022Review
- Multi-Scale Organization of theGenes · 2021Review
- The BEAF-32 Protein Directly Interacts with Z4/putzig and Chriz/Chromator Proteins in Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2021Article
- Overlapping but Distinct Sequences Play Roles in the Insulator and Promoter Activities of theGenetics · 2020Article
- Adaptation of gene loci to heterochromatin in the course of Drosophila evolution is associated with insulator proteins.Scientific reports · 2020Article
- Article
- Transcriptional Silencers in Drosophila Serve a Dual Role as Transcriptional Enhancers in Alternate Cellular Contexts.Molecular cell · 2020Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insulator elements play a role in gene regulation that is potentially linked to nuclear organization. Boundary element-associated factors (BEAFs) 32A and 32B associate with hundreds of sites on Drosophila polytene chromosomes. We hybridized DNA isolated by chromatin immunoprecipitation to genome tiling microarrays to construct a genome-wide map of BEAF binding locations. A distinct difference in the association of 32A and 32B with chromatin was noted. We identified 1,820 BEAF peaks and found that more than 85% were less than 300 bp from transcription start sites. Half are between head-to-head gene pairs. BEAF-associated genes are transcriptionally active as judged by the presence of RNA polymerase II, dimethylated histone H3 K4, and the alternative histone H3.3. Forty percent of these genes are also associated with the polymerase negative elongation factor NELF. Like NELF-associated genes, most BEAF-associated genes are highly expressed. Using quantitative reverse transcription-PCR, we found that the expression levels of most BEAF-associated genes decrease in embryos and cultured cells lacking BEAF. These results provide an unexpected link between BEAF and transcription, suggesting that BEAF plays a role in maintaining most associated promoter regions in an environment that facilitates high transcription levels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.