ArticleNucleic acids research2009
Low-fidelity DNA synthesis by the L979F mutator derivative of Saccharomyces cerevisiae DNA polymerase zeta.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Two distinct Okazaki fragment failure modes dominate mutagenesis in the absence of flap endonuclease 1.Nature communications · 2026Article
- DNA polymerase zeta contributes to heterochromatin replication to prevent genome instability.The EMBO journal · 2021Article
- Compensation for the absence of the catalytically active half of DNA polymerase ε in yeast by positively selected mutations in CDC28.Genetics · 2021Article
- Construction of a highly error-prone DNA polymerase for developing organelle mutation systems.Nucleic acids research · 2020Article
- Genetic Characterization of Three Distinct Mechanisms Supporting RNA-Driven DNA Repair and Modification Reveals Major Role of DNA Polymerase ζ.Molecular cell · 2020Article
- Crossing fitness valleys via double substitutions within codons.BMC biology · 2019Article
- The absence of the catalytic domains of Saccharomyces cerevisiae DNA polymerase ϵ strongly reduces DNA replication fidelity.Nucleic acids research · 2019Article
- Evolutionary switches between two serine codon sets are driven by selection.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Genetic instability in budding and fission yeast-sources and mechanisms.FEMS microbiology reviews · 2015Review
- Eukaryotic DNA polymerase ζ.DNA repair · 2015Review
- Human Pol ε-dependent replication errors and the influence of mismatch repair on their correction.DNA repair · 2013Article
- Review
- DNA polymerase zeta generates clustered mutations during bypass of endogenous DNA lesions in Saccharomyces cerevisiae.Environmental and molecular mutagenesis · 2012Article
- Oncogenic potential is related to activating effect of cancer single and double somatic mutations in receptor tyrosine kinases.Human mutation · 2012Article
- Altered Ig hypermutation pattern and frequency in complementary mouse models of DNA polymerase ζ activity.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- RNase H and postreplication repair protect cells from ribonucleotides incorporated in DNA.Molecular cell · 2012Article
- The major roles of DNA polymerases epsilon and delta at the eukaryotic replication fork are evolutionarily conserved.PLoS genetics · 2011Article
- Lesion bypass by S. cerevisiae Pol ζ alone.DNA repair · 2011Article
- DNA polymerases at the eukaryotic fork-20 years later.Mutation research · 2010Review
- Evolving views of DNA replication (in)fidelity.Cold Spring Harbor symposia on quantitative biology · 2009Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
To probe Pol zeta functions in vivo via its error signature, here we report the properties of Saccharomyces cerevisiae Pol zeta in which phenyalanine was substituted for the conserved Leu-979 in the catalytic (Rev3) subunit. We show that purified L979F Pol zeta is 30% as active as wild-type Pol zeta when replicating undamaged DNA. L979F Pol zeta shares with wild-type Pol zeta the ability to perform moderately processive DNA synthesis. When copying undamaged DNA, L979F Pol zeta is error-prone compared to wild-type Pol zeta, providing a biochemical rationale for the observed mutator phenotype of rev3-L979F yeast strains. Errors generated by L979F Pol zeta in vitro include single-base insertions, deletions and substitutions, with the highest error rates involving stable misincorporation of dAMP and dGMP. L979F Pol zeta also generates multiple errors in close proximity to each other. The frequency of these events far exceeds that expected for independent single changes, indicating that the first error increases the probability of additional errors within 10 nucleotides. Thus L979F Pol zeta, and perhaps wild-type Pol zeta, which also generates clustered mutations at a lower but significant rate, performs short patches of processive, error-prone DNA synthesis. This may explain the origin of some multiple clustered mutations observed in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.