ArticleNucleic acids research2009
Composite RNA aptamers as functional mimics of proteins.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- The Bioactivity of D-/L-Isonucleoside- and 2'-Deoxyinosine-Incorporated Aptamer AS1411s Including DNA Replication/MicroRNA Expression.Molecular therapy. Nucleic acids · 2017Article
- Multivalent Aptamers: Versatile Tools for Diagnostic and Therapeutic Applications.Molecules (Basel, Switzerland) · 2016Review
- An RNA aptamer specific to Hsp70-ATP conformation inhibits its ATPase activity independent of Hsp40.Nucleic acid therapeutics · 2015Article
- General approach for characterizing in vitro selected peptides with protein binding affinity.Analytical chemistry · 2014Article
- In search of novel drug target sites on estrogen receptors using RNA aptamers.Nucleic acid therapeutics · 2014Article
- An optimized streptavidin-binding RNA aptamer for purification of ribonucleoprotein complexes identifies novel ARE-binding proteins.Nucleic acids research · 2014Article
- Nucleic acid aptamers: research tools in disease diagnostics and therapeutics.BioMed research international · 2014Review
- Aptamer in bioanalytical applications.Analytical chemistry · 2011Review
- Knocking down gene function with an RNA aptamer expressed as part of an intron.Nucleic acids research · 2010Article
- Commandeering a biological pathway using aptamer-derived molecular adaptors.Nucleic acids research · 2010Article
- An RNA-based transcription activator derived from an inhibitory aptamer.Nucleic acids research · 2010Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Individual RNA aptamers are often used to modulate the function of their target proteins, and multi-valent aptamers have been constructed to enhance their activity. To expand the utility of aptamers in manipulating and controlling biological processes, here we advance a general method for the design and construction of composite aptamers. The resulting molecular constructs resemble proteins in that they can form specific interactions with three or more different partners and be readily integrated into existing protein regulatory networks. As the first embodiment of this method, we created a tetra-valent aptamer that simultaneously binds to two molecules of the Drosophila protein B52 and two copies of streptavidin, thus mimicking the function of an antibody in immunochemical assays. We demonstrated that the performance of this 'aptabody' rivals that of a monoclonal antibody against B52 in these assays. While this study was performed in vitro and the composite aptamer we made was intended to mimic an existing protein, the same method can be used to accommodate arbitrary combinations of individual aptamers in composite molecular contexts, and these constructs can be delivered into living cells, where they are able to utilize existing cellular infrastructure for their production and processing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.