ArticleBiochemical and biophysical research communications2009
Apical localization of PMCA2w/b is lipid raft-dependent.
Article in Biochemical and biophysical research communications, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Analysis of cholesterol-recognition motifs of the plasma membrane CaJournal of bioenergetics and biomembranes · 2024Article
- Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts.International journal of molecular sciences · 2021Article
- Characterizations of PMCA2-interacting complex and its role as a calcium oxalate crystal-binding protein.Cellular and molecular life sciences : CMLS · 2018Article
- Hypercalcemia induces a proinflammatory phenotype in rat leukocytes and endothelial cells.Journal of physiology and biochemistry · 2013Article
- New advances in urea transporter UT-A1 membrane trafficking.International journal of molecular sciences · 2013Review
- Decreases in plasma membrane Ca²⁺-ATPase in brain synaptic membrane rafts from aged rats.Journal of neurochemistry · 2012Article
- Mature N-linked glycans facilitate UT-A1 urea transporter lipid raft compartmentalization.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Apical localization of PMCA2w/b is enhanced in terminally polarized MDCK cells.Biochemical and biophysical research communications · 2011Article
- Ca2+-signaling, alternative splicing and endoplasmic reticulum stress responses.Neurochemical research · 2011Review
- Early response to ErbB2 over-expression in polarized Caco-2 cells involves partial segregation from ErbB3 by relocalization to the apical surface and initiation of survival signaling.Journal of cellular biochemistry · 2010Article
- Apical scaffolding protein NHERF2 modulates the localization of alternatively spliced plasma membrane Ca2+ pump 2B variants in polarized epithelial cells.The Journal of biological chemistry · 2010Article
- Deletions and mutations in the acidic lipid-binding region of the plasma membrane Ca2+ pump: a study on different splicing variants of isoform 2.The Journal of biological chemistry · 2010Article
- A plasma membrane Ca2+ ATPase isoform at the postsynaptic density.Neuroscience · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Alternative splicing of the first intracellular loop differentially targets plasma membrane calcium ATPase (PMCA) isoform 2 to the apical or basolateral membrane in MDCK cells. To determine if the targeting is affected by lipid interactions, we stably expressed PMCA2w/b and PMCA2z/b in MDCK cells, and analyzed the PMCA distribution by confocal fluorescence microscopy and membrane fractionation. PMCA2w/b showed clear apical and lateral distribution, whereas PMCA2z/b was mainly localized to the basolateral membrane. A significant fraction of PMCA2w/b partitioned into low-density membranes associated with lipid rafts. Depletion of membrane cholesterol by methyl-beta-cyclodextrin resulted in reduced lipid raft association and a striking loss of PMCA2w/b from the apical membrane, whereas the lateral localization of PMCA2z/b remained unchanged. Our data indicate that alternative splicing differentially affects the lipid interactions of PMCA2w/b and PMCA2z/b and that the apical localization of PMCA2w/b is lipid raft-dependent and sensitive to cholesterol depletion.
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