Evidence map›Paper›PMID 19378179›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2009

Static and kinetic site-specific protein-DNA photocrosslinking: analysis of bacterial transcription initiation complexes.

Nikolai Naryshkin, Sergei Druzhinin, Andrei Revyakin, Younggyu Kim, Vladimir Mekler, Richard H Ebright

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Nikolai NaryshkinHHMI, Waksman Institute, Rutgers University, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA.
Sergei Druzhinin
Andrei Revyakin
Younggyu Kim
Vladimir Mekler
Richard H Ebright

Funding

TRANSCRIPTION ACTIVATION BY CAPR01GM041376 · NIGMS · RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK · PI RICHARD H. EBRIGHT · 1989 to 2026
$6.5M
BACTERIAL TRANSCRIPTION COMPLEXESR37GM041376 · NIGMS · RUTGERS, THE STATE UNIV OF N.J. · PI EBRIGHT, RICHARD H. · 2013 to 2022
$5.0M
Howard Hughes Medical InstituteNIGMS NIH HHS GM41376NIGMS NIH HHS R01 GM041376NIGMS NIH HHS R37 GM041376
6 · The paper itself

Abstract

Static site-specific protein-DNA photocrosslinking permits identification of protein-DNA interactions within multiprotein-DNA complexes. Kinetic site-specific protein-DNA photocrosslinking - involving rapid-quench-flow mixing and pulsed-laser irradiation - permits elucidation of pathways and kinetics of formation of protein-DNA interactions within multiprotein-DNA complexes. We present detailed protocols for application of static and kinetic site-specific protein-DNA photocrosslinking to bacterial transcription initiation complexes.

Indexed as

AnimalsCross-Linking ReagentsDNADNA-Directed RNA PolymerasesEscherichia coliKineticsLightMolecular BiologyProteinsTranscription, GeneticCross-Linking ReagentsDNADNA-Directed RNA PolymerasesProteins

Identifiers

PMID19378179
PMCPMC2733221

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.