ReviewMethods in molecular biology (Clifton, N.J.)2009
Design of gene constructs for transgenic maize.
Review in Methods in molecular biology (Clifton, N.J.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Minimizing IP issues associated with gene constructs encoding the Bt toxin - a case study.BMC biotechnology · 2024Article
- Transgenic Improvement for Biotic Resistance of Crops.International journal of molecular sciences · 2022Review
- Three strategies of transgenic manipulation for crop improvement.Frontiers in plant science · 2022Review
- Generation of Transgene-Free Maize Male Sterile Lines Using the CRISPR/Cas9 System.Frontiers in plant science · 2018Article
- Recombinant entomopathogenic agents: a review of biotechnological approaches to pest insect control.World journal of microbiology & biotechnology · 2017Review
- Intron-Mediated Enhancement: A Tool for Heterologous Gene Expression in Plants?Frontiers in plant science · 2016Review
- Synthetic versions of firefly luciferase and Renilla luciferase reporter genes that resist transgene silencing in sugarcane.BMC plant biology · 2014Article
- Comparison of transgene expression in Aedes aegypti generated by mariner Mos1 transposition and ΦC31 site-directed recombination.Insect molecular biology · 2011Article
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Abstract
The first step of any maize transformation project is to select gene expression elements that will make up an effective construct. When designing a gene construct, one must have a full understanding of the different expression elements that are currently available and of the strategies that have been successfully used to overcome obstacles in past. In this chapter, we discuss several major classes of expression elements that have been used for maize transformation, including promoters, introns, and untranslated regions. We also discuss several strategies for further improving transgene expression levels, such as optimization of codon usage, removal of deleterious sequences, addition of signal sequences for subcellular protein targeting, and use of elements to reduce position effects. We hope that this chapter can serve as a general guideline to help researchers, especially beginners in the field, to design a gene construct that will have the maximum potential for gene expression.
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