ArticleMethods in molecular biology (Clifton, N.J.)2009
Positional scanning synthetic combinatorial libraries for substrate profiling.
Article in Methods in molecular biology (Clifton, N.J.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Coupling high-throughput protease enzymology with viral replication reveals biochemical constraints of viral fitness.bioRxiv : the preprint server for biology · 2026Article
- Deep learning guided design of protease substrates.Nature communications · 2026Article
- Engineered MSC-EVs loaded with BDNF-enhancing neuropeptides via a non-disruptive method enhance post-stroke neuroregeneration via intranasal delivery.Journal of nanobiotechnology · 2025Article
- Use of protease substrate specificity screening in the rational design of selective protease inhibitors with unnatural amino acids: Application to HGFA, matriptase, and hepsin.Protein science : a publication of the Protein Society · 2024Article
- The proteasome as a drug target for treatment of parasitic diseases.Advances in parasitology · 2024Review
- Embracing enzyme promiscuity with activity-based compressed biosensing.Cell reports methods · 2023Article
- Multiplex substrate profiling by mass spectrometry for proteases.Methods in enzymology · 2023Article
- Review
- Activity-Based Probes for Proteases Pave the Way to Theranostic Applications.Pharmaceutics · 2022Review
- Universal Access to Protease Chemiluminescent Probes through Solid-Phase Synthesis.Bioconjugate chemistry · 2021Article
- Coumarin as a structural component of substrates and probes for serine and cysteine proteases.Biochimica et biophysica acta. Proteins and proteomics · 2020Review
- Enhanced Antibiofilm Effects of NPolymers · 2020Article
- Synthetic Fluorogenic Peptides Reveal Dynamic Substrate Specificity of Depalmitoylases.Cell chemical biology · 2019Article
- Defining the Determinants of Specificity of Plasmodium Proteasome Inhibitors.Journal of the American Chemical Society · 2018Article
- Synthesis of a HyCoSuL peptide substrate library to dissect protease substrate specificity.Nature protocols · 2017Article
- PEGylated substrates of NSP4 protease: A tool to study protease specificity.Scientific reports · 2016Article
- Article
- Identification of protease exosite-interacting peptides that enhance substrate cleavage kinetics.Biological chemistry · 2012Article
- Directed evolution of protease beacons that enable sensitive detection of endogenous MT1-MMP activity in tumor cell lines.Chemistry & biology · 2011Article
- Biochemical characterization of Plasmodium falciparum dipeptidyl aminopeptidase 1.Molecular and biochemical parasitology · 2011Article
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2 authors.
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Abstract
Determining the preferred substrate cleavage sequence of proteases is an important step toward understanding their roles in cancer development and progression. Knowledge of this sequence can aid in the design of new experimental tools for study as well as aid in the identification of endogenous protease substrates and signaling pathways. Various investigators have demonstrated a number of techniques to uncover these sequences, but most can be very time consuming. We have designed and successfully implemented a complete diverse ACC tetrapeptide positional scanning synthetic combinatorial library that allows for the rapid screening of proteases to determine their preferred residues at positions P1-P4. These sequences can be readily verified through kinetic measurements on single peptide substrates and utilized to further knowledge of the role of proteases in cancer.
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