ArticleCell cycle (Georgetown, Tex.)2009
Prohibitin physically interacts with MCM proteins and inhibits mammalian DNA replication.
Article in Cell cycle (Georgetown, Tex.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Unwinding Helicase MCM Functionality for Diagnosis and Therapeutics of Replication Abnormalities Associated with Cancer: A Review.Molecular diagnosis & therapy · 2024Review
- Prohibitin ligands: a growing armamentarium to tackle cancers, osteoporosis, inflammatory, cardiac and neurological diseases.Cellular and molecular life sciences : CMLS · 2020Review
- A Putative Prohibitin-Calcium Nexus inJournal of diabetes research · 2020Review
- Nuclear Prohibitin3 Maintains Genome Integrity and Cell Proliferation in the Root Meristem through Minichromosome Maintenance 2.Plant physiology · 2019Article
- Prohibitin Shuttles Between Mitochondria and the Nucleus to Control Genome Stability During the Cell Cycle.Plant physiology · 2019Article
- The Human Replicative Helicase, the CMG Complex, as a Target for Anti-cancer Therapy.Frontiers in molecular biosciences · 2018Review
- Adriamycin resistance-associated prohibitin gene inhibits proliferation of human osteosarcoma MG63 cells by interacting with oncogenes and tumor suppressor genes.Oncology letters · 2016Article
- Identification of prohibitin 1 as a potential prognostic biomarker in human pancreatic carcinoma using modified aqueous two-phase partition system combined with 2D-MALDI-TOF-TOF-MS/MS.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- Mammalian lysine histone demethylase KDM2A regulates E2F1-mediated gene transcription in breast cancer cells.PloS one · 2014Article
- S137 phosphorylation of profilin 1 is an important signaling event in breast cancer progression.PloS one · 2014Article
- The Mcm2-7 replicative helicase: a promising chemotherapeutic target.BioMed research international · 2014Review
- Characterization of PHB1 and its role in mitochondrial maturation and yolk platelet degradation during development of Artemia embryos.PloS one · 2014Article
- Prohibitin ligands in cell death and survival: mode of action and therapeutic potential.Chemistry & biology · 2013Review
- Viral and Cellular Components of AAV2 Replication Compartments.The open virology journal · 2013Article
- Involvement of prohibitin upregulation in abrin-triggered apoptosis.Evidence-based complementary and alternative medicine : eCAM · 2012Article
- Prohibitin is overexpressed in Huh-7-HCV and Huh-7.5-HCV cells harboring in vitro transcribed full-length hepatitis C virus RNA.Virology journal · 2011Article
- Dose- and time-dependent expression patterns of zebrafish orthologs of selected E2F target genes in response to serum starvation/replenishment.Molecular biology reports · 2011Article
- The role and therapeutic potential of prohibitin in disease.Biochimica et biophysica acta · 2011Review
- Identification of rep-associated factors in herpes simplex virus type 1-induced adeno-associated virus type 2 replication compartments.Journal of virology · 2010Article
- Relationships between deficits in tissue mass and transcriptional programs after partial hepatectomy in mice.The American journal of pathology · 2009Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
Prohibitin, a tumor suppressor protein, has been shown to repress E2F-mediated transcription and arrest cell cycle progression. while prohibitin has been proposed to regulate cell cycle progression by repressing transcriptional targets of E2F1, it is not clear whether other mechanisms are also involved in mediating the growth arrest. Here we demonstrate that prohibitin can function as a potent inhibitor of DNA replication by interacting with members of Minichromosome maintenance complex of proteins (MCM2-7). The data presented here indicates that prohibitin can physically interact with MCM2, MCM5 and MCM7 in in vitro GST binding assays as well as in MCF-7 cells as seen by immunoprecipitation-western blot experiments. The association was cell cycle dependent, and more pronounced 4-8 hours after serum stimulation of quiescent cells. Prohibitin associated more robustly with MCM2 and MCM5 compared to MCM7, suggesting that prohibitin mainly interacts with the regulatory subunits of the MCM complex. Confirming these results, prohibitin was found to co-localize with MCM2, MCM5 and MCM7 in MCF-7 cells, as seen by double immunofluorescence experiments. Further, Prohibitin strongly inhibited DNA replication in an in vitro replication assay. These results strongly suggest that prohibitin effectively represses replication by interacting with the components of mammalian replication machinery and this might contribute to the growth regulatory properties of prohibitin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.