ArticleApplied and environmental microbiology2009
Effect of pH and temperature on denitrification gene expression and activity in Pseudomonas mandelii.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 193 citations in OpenAlex.
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- Sustainable treatment of nitrate-containing wastewater by an autotrophic hydrogen-oxidizing bacterium.Environmental science and ecotechnology · 2022Article
- Simultaneous Nitrogen Removal and Plant Growth Promotion Using Salt-tolerant Denitrifying Bacteria in Agricultural Wastewater.Microbes and environments · 2022Article
- Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems.Frontiers in microbiology · 2022Article
- Culturable nitrogen-transforming bacteria from sequential sedimentation biofiltration systems and their potential for nutrient removal in urban polluted rivers.Scientific reports · 2021Article
- Simultaneous nitrification-denitrification by phosphate accumulating microorganisms.World journal of microbiology & biotechnology · 2020Review
- Community Composition of Nitrite Reductase Gene Sequences in an Acid Mine Drainage Environment.Microbial ecology · 2020Article
- Nitrogen Removal Performance and Metabolic Pathways Analysis of a Novel Aerobic Denitrifying HalotolerantMicroorganisms · 2020Article
- Effects of Sea Animal Activities on Tundra Soil Denitrification and nirS- and nirK-Encoding Denitrifier Community in Maritime Antarctica.Frontiers in microbiology · 2020Article
- Regenerative stormwater conveyance (RSC) for reducing nutrients in urban stormwater runoff depends upon carbon quantity and quality.The Science of the total environment · 2019Article
- Effects of nitrification inhibitors on gross N nitrification rate, ammonia oxidizers, and NEnvironmental science and pollution research international · 2018Article
- Evaluation of a novel low-carbon to nitrogen- and temperature-tolerant simultaneously nitrifying-denitrifying bacterium and its use in the treatment of river water.RSC advances · 2018Article
- Temporal Changes Rather than Long-Term Repeated Burning Predominately Control the Shift in the Abundance of Soil Denitrifying Community in an Australian Sclerophyll Forest.Microbial ecology · 2017Article
- Temperature-induced changes in treatment efficiency and microbial structure of aerobic granules treating landfill leachate.World journal of microbiology & biotechnology · 2016Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas mandelii liquid cultures were studied to determine the effect of pH and temperature on denitrification gene expression, which was quantified by quantitative reverse transcription-PCR. Denitrification was measured by the accumulation of nitrous oxide (N(2)O) in the headspace in the presence of acetylene. Levels of gene expression of nirS and cnorB at pH 5 were 539-fold and 6,190-fold lower, respectively, than the levels of gene expression for cells grown at pH 6, 7, and 8 between 4 h and 8 h. Cumulative denitrification levels were 28 micromol, 63 micromol, and 22 micromol at pH 6, 7, and 8, respectively, at 8 h, whereas negligible denitrification was measured at pH 5. P. mandelii cells grown at 20 degrees C and 30 degrees C exhibited 9-fold and 94-fold increases in levels of cnorB expression between 0 h and 2 h, respectively, and an average 17-fold increase in levels of nirS gene expression. In contrast, induction of cnorB and nirS gene expression for P. mandelii cells grown at 10 degrees C did not occur in the first 4 h. Levels of cumulative denitrification at 10 h were 6.6 micromol for P. mandelii cells grown at 10 degrees C and 20 degrees C and 30 micromol for cells grown at 30 degrees C. Overall, levels of cnorB and nirS expression were relatively insensitive to pH values over the range of pH 6 to 8 but were substantially reduced at pH 5, whereas gene expression was sensitive to temperature, with induction and time to achieve maximum gene expression delayed as the temperature decreased from 30 degrees C. Low pH and temperature negatively affected denitrification activity.
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