ArticleApplied and environmental microbiology2009
Detection of live Salmonella sp. cells in produce by a TaqMan-based quantitative reverse transcriptase real-time PCR targeting invA mRNA.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Genomic Characterization of an O-Antigen-Deficient, Hydrogen Sulfide-NegativeMicroorganisms · 2026Article
- Comparative evaluation and validation of rapid quantification methods for Mycoplasma hyopneumoniae: development of a PMA-based viability qPCR assay.Scientific reports · 2026Article
- Meta-Transcriptomic Response to Copper Corrosion in Drinking Water Biofilms.Microorganisms · 2025Article
- Quantification of ViableMicroorganisms · 2024Article
- Validation of a simplified small-scale DNA extraction protocol from wine by quantitative real-time PCR.3 Biotech · 2024Article
- Rapid detection ofFood chemistry: X · 2023Article
- Advances in detection methods for viable Salmonella spp.: current applications and challenges.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Review
- Exploration of Infant Food Microbial Composition from Formal and Informal Settings Using Viable Counts and 16S rRNA Gene Amplicon Sequencing in Johannesburg, South Africa.Foods (Basel, Switzerland) · 2023Article
- Mitigating the attachment of Salmonella Infantis on isolated poultry skin with cetylpyridinium chloride.PloS one · 2023Article
- Assessment of pathogens in flood waters in coastal rural regions: Case study after Hurricane Michael and Florence.PloS one · 2023Article
- Internalization of Salmonella in Leafy Greens and Impact on Acid Tolerance.Applied and environmental microbiology · 2022Article
- Genomic Comparison of Eight Closed Genomes of Multidrug-ResistantFrontiers in microbiology · 2022Article
- Development and Evaluation of the Rapid and Sensitive RPA Assays for Specific Detection ofFrontiers in cellular and infection microbiology · 2021Article
- Article
- Application of Short Pre-enrichment, and Double Chemistry Real-Time PCR, Combining Fluorescent Probes and an Intercalating Dye, for Same-Day Detection and Confirmation ofFrontiers in microbiology · 2020Article
- Antimicrobial Efficacy of a Novel Antibiotic-Eluting Injectable Platelet-Rich Fibrin Scaffold against a Dual-Species Biofilm in an Infected Immature Root Canal Model.BioMed research international · 2020Article
- Quantitative LAMP and PCR Detection ofInternational journal of food science · 2020Article
- Closed Genome Sequences of Three Salmonella enterica Strains Belonging to Serovars Saintpaul, Weltevreden, and Thompson, Isolated from Mexico.Microbiology resource announcements · 2019Article
- Quantitative dynamics of Salmonella and E. coli in feces of feedlot cattle treated with ceftiofur and chlortetracycline.PloS one · 2019Article
- Closed Genome Sequence of Salmonella enterica Serovar Richmond Strain CFSAN000191, Obtained with Nanopore Sequencing.Microbiology resource announcements · 2018Article
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Authors and funding
7 authors.
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Abstract
Salmonella enterica contamination in foods is a significant concern for public health. When DNA detection methods are used for analysis of foods, one of the major concerns is false-positive results from the detection of dead cells. To circumvent this crucial issue, a TaqMan quantitative real-time RT-PCR (qRT-PCR) assay with an RNA internal control was developed. invA RNA standards were used to determine the detection limit of this assay as well as to determine invA mRNA levels in mid-exponential-, late-exponential-, and stationary-phase cells. This assay has a detection limit of 40 copies of invA mRNA per reaction. The levels of invA mRNA in mid-exponential-, late-exponential-, and stationary-phase S. enterica cells was approximately 1 copy per 3 CFU, 1 copy per CFU, and 4 copies per 10(3) CFU, respectively. Spinach, tomatoes, jalapeno peppers, and serrano peppers were artificially contaminated with four different Salmonella serovars at levels of 10(5) and less than 10 CFU. These foods were analyzed with qRT-PCR and with the FDA's Bacteriological Analytical Manual Salmonella culture method (W. A. Andrews and T. S. Hammack, in G. J. Jackson et al., ed., Bacteriological analytical manual online, http://www.cfsan.fda.gov/ approximately ebam/bam-5.html, 2007). Comparable results were obtained by both methods. Only live Salmonella cells could be detected by this qRT-PCR assay, thus avoiding the dangers of false-positive results from nonviable cells. False negatives (inhibition of the PCR) were also ruled out through the use of an RNA internal control. This assay allows for the fast and accurate detection of viable Salmonella spp. in spinach, tomatoes, and in both jalapeno and serrano peppers.
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