ArticleJournal of bacteriology2009
In vivo analysis of cobinamide salvaging in Rhodobacter sphaeroides strain 2.4.1.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Flexible Cobamide Metabolism inJournal of bacteriology · 2020Article
- Uneven distribution of cobamide biosynthesis and dependence in bacteria predicted by comparative genomics.The ISME journal · 2019Article
- Rhodobacterales use a unique L-threonine kinase for the assembly of the nucleotide loop of coenzyme BMolecular microbiology · 2018Article
- Construction of Fluorescent Analogs to Follow the Uptake and Distribution of Cobalamin (Vitamin BCell chemical biology · 2018Article
- Identification of specific corrinoids reveals corrinoid modification in dechlorinating microbial communities.Environmental microbiology · 2015Article
- A bioassay for the detection of benzimidazoles reveals their presence in a range of environmental samples.Frontiers in microbiology · 2014Article
- Diversity of cobalamin riboswitches in the corrinoid-producing organohalide respirer Desulfitobacterium hafniense.Journal of bacteriology · 2013Article
- Characterization of four TCE-dechlorinating microbial enrichments grown with different cobalamin stress and methanogenic conditions.Applied microbiology and biotechnology · 2013Article
- The restricted metabolism of the obligate organohalide respiring bacterium Dehalobacter restrictus: lessons from tiered functional genomics.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- Versatility in corrinoid salvaging and remodeling pathways supports corrinoid-dependent metabolism in Dehalococcoides mccartyi.Applied and environmental microbiology · 2012Article
- Structure and mutational analysis of the archaeal GTP:AdoCbi-P guanylyltransferase (CobY) from Methanocaldococcus jannaschii: insights into GTP binding and dimerization.Biochemistry · 2011Article
- The cobinamide amidohydrolase (cobyric acid-forming) CbiZ enzyme: a critical activity of the cobamide remodelling system of Rhodobacter sphaeroides.Molecular microbiology · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The genome of Rhodobacter sphaeroides encodes the components of two distinct pathways for salvaging cobinamide (Cbi), a precursor of adenosylcobalamin (AdoCbl, coenzyme B(12)). One pathway, conserved among bacteria, depends on a bifunctional kinase/guanylyltransferase (CobP) enzyme to convert adenosylcobinamide (AdoCbi) to AdoCbi-phosphate (AdoCbi-P), an intermediate in de novo AdoCbl biosynthesis. The other pathway, of archaeal origin, depends on an AdoCbi amidohydrolase (CbiZ) enzyme to generate adenosylcobyric acid (AdoCby), which is converted to AdoCbi-P by the AdoCbi-P synthetase (CobD) enzyme. Here we report that R. sphaeroides strain 2.4.1 synthesizes AdoCbl de novo and that it salvages Cbi using both of the predicted Cbi salvaging pathways. AdoCbl produced by R. sphaeroides was identified and quantified by high-performance liquid chromatography and bioassay. The deletion of cobB (encoding an essential enzyme of the de novo corrin ring biosynthetic pathway) resulted in a strain of R. sphaeroides that would not grow on acetate in the absence of exogenous corrinoids. The results from a nutritional analysis showed that the presence of either CbiZ or CobP was necessary and sufficient for Cbi salvaging, that CbiZ-dependent Cbi salvaging depended on the presence of CobD, and that CobP-dependent Cbi salvaging occurred in a cbiZ(+) strain. Possible reasons why R. sphaeroides maintains two distinct pathways for Cbi salvaging are discussed.
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