ArticlePlant physiology2009
Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 205 papers, 1 of them a synthesis that pooled it.
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Who cites it
205 citing papers in PubMed, 1 synthesis or guideline pooled it, 487 citations in OpenAlex.
- Systematic Review of Plant Ribosome Heterogeneity and Specialization.Frontiers in plant science · 2020Pooled it
- Complex Regulation of RETINOBLASTOMA-RELATED's Interactions With E2Fs via Phosphorylation.Plant, cell & environment · 2026Article
- A FtsH-Like Zinc Metalloprotease, OsFtsH1, Coordinates Chloroplast Development and Photosystem II Homeostasis in Rice.Rice (New York, N.Y.) · 2026Article
- Article
- Lipidomic profiling of Arabidopsis chloroplast protein phosphatase SLP1 mutants reveals altered diurnal lipid remodeling.BBA advances · 2026Article
- Phylogenetic diversity of light-dependent phosphorylation of Thr78 in Rubisco activase.Journal of experimental botany · 2025Article
- Identification of STN7-associated leaf senescence networks in thylakoid membranes of Arabidopsis.iScience · 2025Article
- Protein Structural Modeling and Transport Thermodynamics Reveal That Plant Cation-Chloride Cotransporters Mediate Potassium-Chloride Symport.International journal of molecular sciences · 2024Article
- Chloroplast ATP synthase: From structure to engineering.The Plant cell · 2024Review
- In Vivo Phosphorylation of the Cytosolic Glucose-6-Phosphate Dehydrogenase Isozyme G6PD6 in Phosphate-ResuppliedPlants (Basel, Switzerland) · 2023Article
- Dynamics of ribosome composition and ribosomal protein phosphorylation in immune signaling in Arabidopsis thaliana.Nucleic acids research · 2023Article
- The Replicative DnaE Polymerase ofLife (Basel, Switzerland) · 2023Article
- Characterization of mutants deficient in N-terminal phosphorylation of the chloroplast ATP synthase subunit β.Plant physiology · 2023Article
- Redox Signaling in Plant Heat Stress Response.Antioxidants (Basel, Switzerland) · 2023Review
- Characterization of the preferred cation cofactors of chloroplast protein kinases in Arabidopsis thaliana.FEBS open bio · 2023Article
- AtSWEET11 and AtSWEET12 transporters function in tandem to modulate sugar flux in plants.Plant direct · 2023Article
- The MAPK-Alfin-like 7 module negatively regulates ROS scavenging genes to promote NLR-mediated immunity.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Glycine-Induced Phosphorylation Plays a Pivotal Role in Energy Metabolism in Roots and Amino Acid Metabolism in Leaves of Tea Plant.Foods (Basel, Switzerland) · 2023Article
- CGL160-mediated recruitment of the coupling factor CF1 is required for efficient thylakoid ATP synthase assembly, photosynthesis, and chloroplast development in Arabidopsis.The Plant cell · 2023Article
- Energetic considerations for engineering novel biochemistries in photosynthetic organisms.Frontiers in plant science · 2023Review
145 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized the phosphoproteome of Arabidopsis (Arabidopsis thaliana) seedlings using high-accuracy mass spectrometry and report the identification of 1,429 phosphoproteins and 3,029 unique phosphopeptides. Among these, 174 proteins were chloroplast phosphoproteins. Motif-X (motif extractor) analysis of the phosphorylation sites in chloroplast proteins identified four significantly enriched kinase motifs, which include casein kinase II (CKII) and proline-directed kinase motifs, as well as two new motifs at the carboxyl terminus of ribosomal proteins. Using the phosphorylation motifs as a footprint for the activity of a specific kinase class, we connected the phosphoproteins with their putative kinases and constructed a chloroplast CKII phosphorylation network. The network topology suggests that CKII is a central regulator of different chloroplast functions. To provide insights into the dynamic regulation of protein phosphorylation, we analyzed the phosphoproteome at the end of day and end of night. The results revealed only minor changes in chloroplast kinase activities and phosphorylation site utilization. A notable exception was ATP synthase beta-subunit, which is found phosphorylated at CKII phosphorylation sites preferentially in the dark. We propose that ATP synthase is regulated in cooperation with 14-3-3 proteins by CKII-mediated phosphorylation of ATP synthase beta-subunit in the dark.
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