Trial reportAmerican journal of obstetrics and gynecology2009
Microfluidic digital PCR enables rapid prenatal diagnosis of fetal aneuploidy.
Trial report in American journal of obstetrics and gynecology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 106 citations in OpenAlex.
- A Fetal Fraction Optimized 106-Plex Digital PCR Assay for Non-Invasive Prenatal Testing of Fetal Trisomy.Diagnostics (Basel, Switzerland) · 2026Article
- Review
- Age-related cardiovascular disease and mosaic hematopoietic loss of the Y chromosome.The journal of cardiovascular aging · 2025Article
- Urinary Biomarkers and Point-of-Care Urinalysis Devices for Early Diagnosis and Management of Disease: A Review.Biomedicines · 2023Review
- The Influence of Maternal Cell Contamination on Fetal Aneuploidy Detection Using Chip-Based Digital PCR Testing.Diagnostics (Basel, Switzerland) · 2021Article
- Clinical Validation of Novel Chip-Based Digital PCR Platform for Fetal Aneuploidies Screening.Diagnostics (Basel, Switzerland) · 2021Article
- Fabrication and Applications of Microfluidic Devices: A Review.International journal of molecular sciences · 2021Review
- Microfluidic chips: recent advances, critical strategies in design, applications and future perspectives.Microfluidics and nanofluidics · 2021Review
- Next-generation sequencing of 35 RHD variants in 16 253 serologically D- pregnant women in the Finnish population.Blood advances · 2020Article
- Single-Cell-Based Digital PCR Detection and Association of Shiga Toxin-Producing Escherichia coli Serogroups and Major Virulence Genes.Journal of clinical microbiology · 2020Article
- Chip-Based Digital PCR Approach Provides A Sensitive and Cost-Effective Single-Day Screening Tool for Common Fetal Aneuploidies-A Proof of Concept Study.International journal of molecular sciences · 2019Article
- Current, Emerging, and Future Applications of Digital PCR in Non-Invasive Prenatal Diagnosis.Molecular diagnosis & therapy · 2018Review
- Microfluidics in reproductive biology: applying lab-on-a-chip technologies to assisted reproduction.Molecular human reproduction · 2017Article
- Applicability of digital PCR to the investigation of pediatric-onset genetic disorders.Biomolecular detection and quantification · 2016Review
- Three-color crystal digital PCR.Biomolecular detection and quantification · 2016Article
- The μSCAPE System: 3-Dimensional Profiling of Microfluidic Architectural Features Using a Flatbed Scanner.Scientific reports · 2016Article
- Automatic sequential fluid handling with multilayer microfluidic sample isolated pumping.Biomicrofluidics · 2015Article
- Direct and rapid mass spectral fingerprinting of maternal urine for the detection of Down syndrome pregnancy.Clinical proteomics · 2015Article
- Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering.Nature nanotechnology · 2014Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveThe purpose of this study was to demonstrate that digital polymerase chain reaction (PCR) enables rapid, allele independent molecular detection of fetal aneuploidy. STUDY
designTwenty-four amniocentesis and 16 chorionic villus samples were used for microfluidic digital PCR analysis. Three thousand and sixty PCR reactions were performed for each of the target chromosomes (X, Y, 13, 18, and 21), and the number of single molecule amplifications was compared to a reference. The difference between target and reference chromosome counts was used to determine the ploidy of each of the target chromosomes.
resultsDigital PCR accurately identified all cases of fetal trisomy (3 cases of trisomy 21, 3 cases of trisomy 18, and 2 cases of triosmy 13) in the 40 specimens analyzed. The remaining specimens were determined to have normal ploidy for the chromosomes tested.
conclusionMicrofluidic digital PCR allows detection of fetal chromosomal aneuploidy utilizing uncultured amniocytes and chorionic villus tissue in less than 6 hours.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.