ArticleJournal of immunological methods2009
Human CD1 dimeric proteins as indispensable tools for research on CD1-binding lipids and CD1-restricted T cells.
Article in Journal of immunological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- An immunostimulatory glycolipid that blocks SARS-CoV-2, RSV, and influenza infections in vivo.Nature communications · 2023Article
- Reciprocal Crosstalk between Dendritic Cells and Natural Killer T Cells: Mechanisms and Therapeutic Potential.Frontiers in immunology · 2017Review
- A potent adjuvant effect of a CD1d-binding NKT cell ligand in human immune system mice.Expert review of vaccines · 2017Article
- Immunotherapeutic strategies targeting natural killer T cell responses in cancer.Immunogenetics · 2016Review
- The tumor antigen N-glycolyl-GM3 is a human CD1d ligand capable of mediating B cell and natural killer T cell interaction.Cancer immunology, immunotherapy : CII · 2016Article
- Effects of Invariant NKT Cells on Parasite Infections and Hygiene Hypothesis.Journal of immunology research · 2016Review
- Invariant natural killer T cells generated from human adult hematopoietic stem-progenitor cells are poly-functional.Cytokine · 2015Article
- Development of a qPCR method to rapidly assess the function of NKT cells.Journal of immunological methods · 2014Article
- Lipid antigens in immunity.Biological chemistry · 2014Review
- Nanoengineering approaches to the design of artificial antigen-presenting cells.Nanomedicine (London, England) · 2013Review
- Generation of Mouse iNKT Cell Lines.Bio-protocol · 2013Article
- Generation of Human iNKT Cell Lines.Bio-protocol · 2013Article
- Artificial antigen presenting cell (aAPC) mediated activation and expansion of natural killer T cells.Journal of visualized experiments : JoVE · 2012Article
- Connecting the dots: artificial antigen presenting cell-mediated modulation of natural killer T cells.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2012Review
- Molecular identification of GD3 as a suppressor of the innate immune response in ovarian cancer.Cancer research · 2012Article
- Design of a potent CD1d-binding NKT cell ligand as a vaccine adjuvant.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
Abstract
Antigen presenting molecules play an important role in both innate and adoptive immune responses by priming and activating T cells. Among them, CD1 molecules have been identified to present both exogenous and endogenous lipid antigens to CD1-restricted T cells. The involvement of CD1-restricted T cells in autoimmune diseases and in defense against infectious diseases, however, remains largely unknown. Identifying novel antigenic lipids that bind to CD1 molecules and understanding the role of CD1-restricted T cells should lead to the successful development of vaccines, because the lipids can be used as antigens and also as adjuvants. In this paper, we have constructed functional recombinant human CD1 dimeric proteins and established a competitive ELISA assay to measure the lipid binding to CD1 molecules using the CD1 dimers. By using the competitive ELISA assay, we were able to show that the lipid extracts from murine malaria parasites can actually be loaded onto CD1 molecules. In addition, we have demonstrated that artificial antigen-presenting cells, which consist of magnetic beads coated with CD1d dimer and anti-CD28 antibody, stimulated and expanded human invariant NKT cells as efficiently as autologous immature DCs. A set of the tools presented in the current study should be valuable for screening various CD1 molecule-binding lipid antigens and for isolating CD1-restricted T cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.