ArticleFEMS microbiology letters2009
Molecular characterization and identification of proteins regulated by Hfq in Neisseria meningitidis.
Article in FEMS microbiology letters, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- The impact of Hfq-mediated sRNA-mRNA interactome on the virulence of enteropathogenicScience advances · 2021Article
- The minimal meningococcal ProQ protein has an intrinsic capacity for structure-based global RNA recognition.Nature communications · 2020Article
- Regulation ofFEBS open bio · 2017Article
- The primary transcriptome of Neisseria meningitidis and its interaction with the RNA chaperone Hfq.Nucleic acids research · 2017Article
- The Hfq regulon ofFEBS open bio · 2017Article
- Article
- UnravelingF1000Research · 2017Review
- Loop structures in the 5' untranslated region and antisense RNA mediate pilE gene expression in Neisseria gonorrhoeae.Microbiology (Reading, England) · 2016Article
- Characterization of a novel antisense RNA in the major pilin locus of Neisseria meningitidis influencing antigenic variation.Journal of bacteriology · 2015Article
- Involvement of Neisseria meningitidis lipoprotein GNA2091 in the assembly of a subset of outer membrane proteins.The Journal of biological chemistry · 2014Article
- Edwardsiella tarda Hfq: impact on host infection and global protein expression.Veterinary research · 2014Article
- Mapping Hfq-RNA interaction surfaces using tryptophan fluorescence quenching.Nucleic acids research · 2014Article
- Transcriptional profiling of Neisseria meningitidis interacting with human epithelial cells in a long-term in vitro colonization model.Infection and immunity · 2013Article
- The zinc-responsive regulon of Neisseria meningitidis comprises 17 genes under control of a Zur element.Journal of bacteriology · 2012Article
- Structural mechanism of Staphylococcus aureus Hfq binding to an RNA A-tract.Nucleic acids research · 2012Article
- Analysis of the regulated transcriptome of Neisseria meningitidis in human blood using a tiling array.Journal of bacteriology · 2012Article
- Crystal structure of Hfq from Bacillus subtilis in complex with SELEX-derived RNA aptamer: insight into RNA-binding properties of bacterial Hfq.Nucleic acids research · 2012Article
- Deep sequencing whole transcriptome exploration of the σE regulon in Neisseria meningitidis.PloS one · 2011Article
- Role of Hfq in iron-dependent and -independent gene regulation in Neisseria meningitidis.Microbiology (Reading, England) · 2010Article
- Identification of a novel anti-sigmaE factor in Neisseria meningitidis.BMC microbiology · 2010Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Hfq is a highly conserved pleiotropically acting prokaryotic RNA-binding protein involved in the post-transcriptional regulation of many stress-responsive genes by small RNAs. In this study, we show that Hfq of the strictly human pathogen Neisseria meningitidis is involved in the regulation of expression of components involved in general metabolic pathways, iron metabolism and virulence. A meningococcal hfq deletion strain (H44/76Deltahfq) is impaired in growth in nutrient-rich media and does not grow at all in nutrient-limiting medium. The growth defect was complemented by expression of hfq in trans. Using proteomics, the expression of 28 proteins was found to be significantly affected upon deletion of hfq. Of these, 20 proteins are involved in general metabolism, among them seven iron-responsive genes. Two proteins (PilE, TspA) are involved in adherence to human cells, a step crucial for the onset of disease. One of the differentially expressed proteins, GdhA, was identified as an essential virulence factor for establishment of sepsis in an animal model, studied earlier. These results show that in N. meningitidis Hfq is involved in the regulation of a variety of components contributing to the survival and establishment of meningococcal disease.
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