ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2009
Induction of DNA damage response by the supravital probes of nucleic acids.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 69 citations in OpenAlex.
- Tight association of autophagy and cell cycle in leukemia cells.Cellular & molecular biology letters · 2022Article
- DNA Dyes-Highly Sensitive Reporters of Cell Quantification: Comparison with Other Cell Quantification Methods.Molecules (Basel, Switzerland) · 2021Review
- Simultaneous label-free live imaging of cell nucleus and luminescent nanodiamonds.Scientific reports · 2020Article
- Measuring DNA content in live cells by fluorescence microscopy.Cell division · 2018Article
- Sodium fluoride induces apoptosis in mouse splenocytes by activating ROS-dependent NF-κB signaling.Oncotarget · 2017Article
- A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry.Journal of visualized experiments : JoVE · 2017Article
- Cell cycle staging of individual cells by fluorescence microscopy.Nature protocols · 2015Article
- Mitochondrial staining allows robust elimination of apoptotic and damaged cells during cell sorting.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2014Article
- DNA damage signaling, impairment of cell cycle progression, and apoptosis triggered by 5-ethynyl-2'-deoxyuridine incorporated into DNA.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Article
- Daunomycin, an antitumor DNA intercalator, influences histone-DNA interactions.Cancer biology & therapy · 2013Article
- From seeing to believing: labelling strategies for in vivo cell-tracking experiments.Interface focus · 2013Article
- Real-time cell viability assays using a new anthracycline derivative DRAQ7®.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Article
- Calcein-effluxing human colon cancer cells are enriched for self-renewal capacity and depend on β-catenin.Oncotarget · 2013Article
- Biomarkers of cell senescence assessed by imaging cytometry.Methods in molecular biology (Clifton, N.J.) · 2013Article
- BubR1 is modified by sumoylation during mitotic progression.The Journal of biological chemistry · 2012Article
- Cell fixation in zinc salt solution is compatible with DNA damage response detection by phospho-specific antibodies.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2011Article
- Label-free 3D visualization of cellular and tissue structures in intact muscle with second and third harmonic generation microscopy.PloS one · 2011Article
- Cell synchronization by inhibitors of DNA replication induces replication stress and DNA damage response: analysis by flow cytometry.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Analysis of individual molecular events of DNA damage response by flow- and image-assisted cytometry.Methods in cell biology · 2011Review
- In vitro generation of Plasmodium falciparum ookinetes.The American journal of tropical medicine and hygiene · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
The aim of this study was to assess the potential DNA damage response (DDR) to four supravitally used biomarkers Hoechst 33342 (Ho 42), DRAQ5, DyeCycle Violet (DCV), and SYTO 17. A549 cells were exposed to these biomarkers at concentrations generally applied to live cells and their effect on histone H2AX (Ser 139), p53 (Ser15), ATM (Ser1981), and Chk2 (Thr68) phosphorylation was assessed using phospho-specific Abs. Short-term treatment with Ho 42 led to modest degree of ATM activation with no evidence of H2AX, Chk2, or p53 phosphorylation. However, pronounced ATM, Chk2, and p53 phosphorylation and perturbed G(2) progression were seen after 18 h. While short-term treatment with DRAQ5 induced ATM activation with no effect on H2AX, Chk2, and p53, dramatic changes marked by a high degree of H2AX, ATM, Chk2, and p53 phosphorylation, all occurring predominantly in S phase cells, and a block in cell cycle progression, were seen after 18 h exposure. These changes suggest that the DRAQ5-induced DNA lesions may become converted into double-strand DNA breaks during replication. Exposure to DCV also led to an increase in the level of activated ATM and Chk2 as well as of phosphorylated p53 and accumulation of cells in G(2)M and S phase. Exposure to SYTO 17 had no significant effect on any of the measured parameters. The data indicate that supravital use of Ho 42, DRAQ5, and DCV induces various degrees of DDR, including activation of ATM, Chk2 and p53, which may have significant consequences on regulatory cell cycle pathways and apoptosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.