ArticleMolecular biology reports2010
GmRFP1 encodes a previously unknown RING-type E3 ubiquitin ligase in Soybean (Glycine max).
Article in Molecular biology reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 40 citations in OpenAlex.
- Genome-wide identification of the ATL gene family and their expression analysis in sweetpotato and its two diploid relatives.BMC plant biology · 2026Article
- Screening for GmRCD1-Interacting Proteins in Glycine Max and Characterization of the GmRCD1-GmNAC058 Interaction.International journal of molecular sciences · 2025Article
- A tree peony RING-H2 finger protein, PsATL33, plays an essential role in cold-induced bud dormancy release by regulating gibberellin content.Frontiers in plant science · 2024Article
- The soybean ubiquitin-proteasome system: Current knowledge and future perspective.The plant genome · 2023Review
- Combined QTL mapping and RNA-Seq pro-filing reveal candidate genes related to low-temperature tolerance in maize.Molecular breeding : new strategies in plant improvement · 2022Article
- Article
- Evolutionary and Regulatory Pattern Analysis of Soybean CaFrontiers in plant science · 2022Article
- RING Zinc Finger Proteins in Plant Abiotic Stress Tolerance.Frontiers in plant science · 2022Review
- Endoplasmic reticulum-related E3 ubiquitin ligases: Key regulators of plant growth and stress responses.Plant communications · 2021Review
- Interaction ofFrontiers in plant science · 2021Article
- Genome-Wide Association Analyses Reveal Genomic Regions Controlling Canopy Wilting in Soybean.G3 (Bethesda, Md.) · 2020Article
- Genome-wide analysis of genes encoding core components of the ubiquitin system in soybean (Glycine max) reveals a potential role for ubiquitination in host immunity against soybean cyst nematode.BMC plant biology · 2018Article
- RING E3 ligases: key regulatory elements are involved in abiotic stress responses in plants.BMB reports · 2017Review
- Genome-wide characterisation and expression profile of the grapevine ATL ubiquitin ligase family reveal biotic and abiotic stress-responsive and development-related members.Scientific reports · 2016Article
- Overexpression of a soybean ariadne-like ubiquitin ligase gene GmARI1 enhances aluminum tolerance in Arabidopsis.PloS one · 2014Article
- Genome-wide analysis of soybean HD-Zip gene family and expression profiling under salinity and drought treatments.PloS one · 2014Article
- The RING-H2 finger gene 1 (RHF1) encodes an E3 ubiquitin ligase and participates in drought stress response in Nicotiana tabacum.Genetica · 2013Article
- The prolific ATL family of RING-H2 ubiquitin ligases.Plant signaling & behavior · 2012Review
- Expression, characterization and 2,4,6-trichlorophenol degradation of laccase from Monilinia fructigena.Molecular biology reports · 2012Article
- Proteomics-based analysis of novel genes involved in response toward soybean mosaic virus infection.Molecular biology reports · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RING-finger proteins with E3 ubiquitin ligase activity play important roles in the regulation of plant growth and development. In this study, a cDNA clone encoding a novel RING-finger protein, designated as GmRFP1, was isolated and characterized from soybean. GmRFP1 was an intronless gene encoding a predicted protein product of 392 amino acid residues with a molecular mass of ~43 kDa. The protein contained a RING-H2 motif and an N-terminal transmembrane domain. The transcript was observed in all detected organs and was up-regulated by abscisic acid (ABA) and salt stress, but down-regulated by cold and drought treatments. We further expressed and purified both wild type and mutant version of GmRFP1 in E. coli. In vitro assays showed that the purified GmRFP1 induced the formation of polyubiquitin chains while mutation within the RING-finger region abolished the ubiquitination activity. These findings suggest that GmRFP1 is a previously unknown E3 ubiquitin ligase in soybean and that the RING domain is required for its activity. It may play unappreciated roles in ABA signaling and stress responses via mediating the ubiquitination and degradation of target proteins through the ubiquitin-proteasome pathway.
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